CHEMICAL COUPLING OF YEAST HEXOKINASE TO SEPHADEX

被引：8
作者：
BOHNENSACK, R
AUGUSTIN, W
HOFMANN, E
机构：
[1] Physiologisch-chemisches Institut der Medizinischen Akademie Magdeburg, Germany (Democratic Republic
[2] Physiologisch-chemisches Institut der Karl-Marx-Universität, 701 Leipzig, Germany (Democratic Republic
来源：
EXPERIENTIA | 1969年 / 25卷 / 04期
关键词：
D O I：
10.1007/BF01899906
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Yeast hexokinase was coupled to Sephadex by means of cyanogen bromide. The activity of the fixed enzyme was 2% of that before coupling. The activity of the noncoupled part of the enzyme was also decreased to the same degree. This decrease cannot be caused by the coupling reaction itself. The Michaelis-constants and the pH-activity curve of the coupled enzyme do not differ significantly from that of the free enzyme. © 1969 Birkhäuser-Verlag.
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页码：348 / +
页数：1
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