CORALLINE SHAPE OF THE BACTERIAL NUCLEOID AFTER CRYOFIXATION

被引：46

作者：

BOHRMANN, B ^{[1
]}

VILLIGER, W ^{[1
]}

JOHANSEN, R ^{[1
]}

KELLENBERGER, E ^{[1
]}

机构：

[1] UNIV BASEL,BIOCTR,DEPT MICROBIOL,KLINGELBERGSTR 70,CH-4056 BASEL,SWITZERLAND

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 10期

关键词：

D O I：

10.1128/JB.173.10.3149-3158.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new procedure of immunostaining sections of cryofixed and freeze-substituted Escherichia coli shows that DNA extends from its bulk into small ribosome-free spaces throughout the cytoplasm, resulting in a coralline-shaped nucleoid. Low-resolution imaging of a bacterium reconstructed from serial sections demonstrated that the small excrescencies are not resolved. The resulting photograph shows the same features as phase-contrast light micrographs.

引用

页码：3149 / 3158

页数：10

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