ANALYSIS OF PHENPROCOUMON AND ITS HYDROXYLATED AND CONJUGATED METABOLITES IN HUMAN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AFTER SOLID-PHASE EXTRACTION

被引：16
|
作者：
EDELBROEK, PM
VANKEMPEN, GMJ
HESSING, TJ
DEWOLFF, FA
机构：
[1] STATE UNIV LEIDEN HOSP,TOXICOL LAB,2333 AA LEIDEN,NETHERLANDS
[2] PSYCHIAT HOSP ENDEGEEST,BIOCHEM LAB,OEGSTGEEST,NETHERLANDS
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 530卷 / 02期
关键词：
D O I：
10.1016/S0378-4347(00)82337-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The anticoagulant phenprocoumon is mainly metabolized in humans to hydroxylated metabolites and their glucuronides. A method is described for the determination of phenprocoumon, 4′-hydroxyphenprocoumon, 6-hydroxyphenprocoumon, 7-hydroxyphenprocoumon, and their glucuronide and sulphate conjugates in human urine. Reversed-phase high-performance liquid chromatography is performed after selective extraction with disposable quaternary amine columns of untreated, and β-glucuronidase- or sulphatase-treated urine samples. Urinary excretion data are presented for total, glucuronidated, sulphated and free phenprocoumon, 4′-hydroxyphenprocoumon, 6-hydroxyphenprocoumon and 7-hydroxyphenprocoumon in twelve patients after an average daily dosage of 1.3-4.2 mg phenprocoumon. © 1990.
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页码：347 / 358
页数：12
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