REGENERATION OF WHOLE PLANTS FROM CALLUS-CULTURE OF DIVERSE GENETIC LINES OF PISUM-SATIVUM-L

Sixteen genetic lines of peas were screened for their ability to regenerate whole plants from callus cultures. Epicotyl sections from germinating seeds were placed on callus-inducing medium; the resulting callus was subcultured monthly and was tested every other month for its regeneration ability. Six lines were found that would regenerate after 2 months' growth as callus. Four of these continued to regenerate after 4 months and, of these, two after 6 months. The cultivars Frosty" and "Alaska" were among the lines that would not regenerate at all. © 1979 Springer-Verlag."