COUNTERSELECTION OF GATC SEQUENCES IN ENTEROBACTERIOPHAGES BY THE COMPONENTS OF THE METHYL-DIRECTED MISMATCH REPAIR SYSTEM

被引:26
作者
DESCHAVANNE, P
RADMAN, M
机构
[1] Laboratoire de Mutagénèse, Institut J. Monod, Paris Cedex 05, 75251
关键词
GATC SEQUENCE; COUNTERSELECTION; MISMATCH REPAIR SYSTEM; ENTEROBACTERIOPHAGES; ESCHERICHIA-COLI;
D O I
10.1007/BF02193626
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Weak to severe deficit of GATC sequences in the DNA of enterobacteriophages appears to be correlated with their undermethylation during growth in dam+ (GATC ade-methylase) bacteria. This observation is corroborated by the sequence analysis showing no evidence for site-specific mutagenicity of 6meAde. The MutH protein of the methyl-directed mismatch repair system recognizes and cleaves the undermethylated GATC sequences in the course of mismatch repair. To enquire whether the MutH function of the methyl-directed mismatch repair system participates in counterselection of GATC sequences in enterobacteriophages, we have studied the yield of bacteriophage PHI-X174 containing either 0, 1, or 2 GATC sequences, in wild type, dam, and mut (H, L, S, U) Escherichia coli. Following transfection with unmethylated DNA containing two GATC sequences, a net decrease in the yield of infective particles was observed in all bacterial mutH+ dam- strains, whereas no detectable decrease was observed in bacteria infected by DNA without GATC sequence. This effect of the MutH function is maximum in wild type and mutL and mutS bacteria whereas the effect is not significant in mutU bacteria, suggesting an interaction of the helicase II with the MutH protein. However, in dam+ bacteria, the presence of GATC sequences leads to an increased yield of infective particles. The effect of GATC sequence and its Dam methylation system on phage yield in mutH-bacteria reveals that methylated GATC sequences are advantageous to the phage. These results suggest that the methyl-directed mismatch repair system, and in particular its MutH protein, may have participated in severe counterselection of GATC sequences from enterobacteriophages, presumably by DNA cleavage or by interfering with DNA replication or packaging when GATC sequences are undermethylated. Coevolution of the Dam and MutH proteins could then account for the loss of GATC sequences from DNA of bacteriophages growing in dam+ hosts.
引用
收藏
页码:125 / 132
页数:8
相关论文
共 51 条
[1]   EVIDENCE THAT ESCHERICHIA-COLI VIRUS-T1 INDUCES A DNA METHYLTRANSFERASE [J].
AUER, B ;
SCHWEIGER, M .
JOURNAL OF VIROLOGY, 1984, 49 (02) :588-590
[2]   INHIBITION OF THE TYPE-I RESTRICTION-MODIFICATION ENZYMES ECOB AND ECOK BY THE GENE O.3 PROTEIN OF BACTERIOPHAGE-T7 [J].
BANDYOPADHYAY, PK ;
STUDIER, FW ;
HAMILTON, DL ;
YUAN, R .
JOURNAL OF MOLECULAR BIOLOGY, 1985, 182 (04) :567-578
[3]   DNA ADENINE METHYLATION OF GATC SEQUENCES APPEARED RECENTLY IN THE ESCHERICHIA-COLI LINEAGE [J].
BARBEYRON, T ;
KEAN, K ;
FORTERRE, P .
JOURNAL OF BACTERIOLOGY, 1984, 160 (02) :586-590
[4]   TRANSFECTION OF ENTEROBACTERIACEAE AND ITS APPLICATIONS [J].
BENZINGER, R .
MICROBIOLOGICAL REVIEWS, 1978, 42 (01) :194-236
[5]   CPG-RICH ISLANDS AND THE FUNCTION OF DNA METHYLATION [J].
BIRD, AP .
NATURE, 1986, 321 (6067) :209-213
[6]   SOS MUTATOR EFFECT IN ESCHERICHIA-COLI MUTANTS DEFICIENT IN MISMATCH CORRECTION [J].
CAILLETFAUQUET, P ;
MAENHAUTMICHEL, G ;
RADMAN, M .
EMBO JOURNAL, 1984, 3 (04) :707-712
[7]  
CLAVERYS JP, 1986, MICROBIOL REV, V50, P133, DOI 10.1128/MMBR.50.2.133-165.1986
[8]   CYTOSINE METHYLATION AND THE FATE OF CPG DINUCLEOTIDES IN VERTEBRATE GENOMES [J].
COOPER, DN ;
KRAWCZAK, M .
HUMAN GENETICS, 1989, 83 (02) :181-188
[9]   DNA METHYLATION AND CPG SUPPRESSION [J].
COOPER, DN ;
GERBERHUBER, S .
CELL DIFFERENTIATION, 1985, 17 (03) :199-205
[10]   EUKARYOTIC DNA METHYLATION [J].
COOPER, DN .
HUMAN GENETICS, 1983, 64 (04) :315-333