MAPPING OF THE MAB73 EPITOPE ON AD2 E1A PROTEINS WITH RANDOM PEPTIDE LIBRARIES AND DELETION MUTANTS

被引：12

作者：

ARSENAULT, H ^{[1
]}

WEBER, JM ^{[1
]}

机构：

[1] UNIV SHERBROOKE, FAC MED, DEPT MICROBIOL, SHERBROOKE J1H 5N4, PQ, CANADA

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 114卷 / 01期

基金：

英国医学研究理事会;

关键词：

ADENOVIRUS E1A; EPITOPE; MONOCLONAL ANTIBODY MAB73; PEPTIDE LIBRARY; NUCLEAR TRANSPORT SIGNAL;

D O I：

10.1111/j.1574-6968.1993.tb06547.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

mAb73, a monoclonal antibody against adenoviruses type 2 and 5 E1A, recognises an epitope within the C-terminal part of this protein. To identify the epitope we used random peptide libraries expressed on the surface of filamentous phages (Fd, M13). We found a consensus sequence homologous to the nuclear transport signal KRPRP at the C-terminus of Ad2 and Ad5 E1A. An E1A mutant deleted for these residues failed to be immunoprecipitated by mAb73, confirming that the nuclear transport signal of E1A is the epitope recognised by mAb73.

引用

页码：37 / 40

页数：4

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