THE CATALYTIC DOMAIN OF HUMAN HEPATITIS-DELTA VIRUS-RNA - A PROTON NUCLEAR-MAGNETIC-RESONANCE STUDY

被引：5
作者：
LEE, BS
WU, HN
HUANG, TH
机构：
[1] INST BIOMED SCI, TAIPEI 11529, TAIWAN
[2] ACAD SINICA, INST MOL BIOL, TAIPEI 11529, TAIWAN
来源：
FEBS LETTERS | 1993年 / 324卷 / 03期
关键词：
RIBOZYME; RNA; STRUCTURE; NMR; HEPATITIS DELTA VIRUS;
D O I：
10.1016/0014-5793(93)80138-K
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have obtained and analyzed the 600 MHz proton NMR spectra of a 74-mer RNA derived from the catalytic domain of hepatitis delta virus genomic RNA (HDV RNA) to determine its secondary structure. Deconvolution of the NMR spectrum obtained at 32-degrees-C indicates that part of the 74-mer RNA molecule may exist in multiple conformations in equilibrium. The major conformer contains two A-U base pairs and 14 +/- 2 G-C base pairs. It appears to contain no standard G-U base pairs. Our NMR melting study suggests that this conformer has at least two stem-loop regions. One of the regions has been identified to be a tetra-loop. We have assigned five imino proton resonances of the tetra-loop stem. Our data is consistent with the pseudoknot model of Perrotta and Been.
引用
收藏
页码：296 / 300
页数：5
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