LANGMUIR-BLODGETT MONOLAYER FILMS OF THE RHODOPSEUDOMONAS-VIRIDIS REACTION CENTER - DETERMINATION OF THE ORDER OF THE HEMES IN THE CYTOCHROME-C SUBUNIT .2.

被引：50

作者：

ALEGRIA, G

DUTTON, PL

机构：

[1] Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelpha, PA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1057卷 / 02期

关键词：

LANGMUIR-BLODGETT FILM; BACTERIAL REACTION CENTER; PHOTOSYNTHETIC BACTERIUM; CHROMATOPHORE; LINEAR DICHROISM; (RHODOPSEUDOMONAS-VIRIDIS);

D O I：

10.1016/S0005-2728(05)80108-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Langmuir-Blodgett (LB) film technique has been applied to produce oriented and photo-active films of isolated reaction center cytochrome c complexes (RC-cyt c) and chromatophore membranes from the photosynthetic bacterium Rhodopseudomonas viridis. Linear dichroism (LD) and redox potentiometry have been used to identify the four cytochrome c hemes of the RC-cyt c complex. Resolved angular orientations of the four hemes in LB films of both isolated RC-cyt-c complexes and of chromatophore membranes permit the reduction of the 24 possible arrangements to two. Additional structural and functional information from other sources allows us to propose a model which best accounts for all the available experimental data.

引用

页码：258 / 272

页数：15

共 33 条

[1]

ALEGRIA G, 1990, BIOPHYS J, V57, pA571

[2] LANGMUIR-BLODGETT MONOLAYER FILMS OF BACTERIAL PHOTOSYNTHETIC MEMBRANES AND ISOLATED REACTION CENTERS - PREPARATION, SPECTROPHOTOMETRIC AND ELECTROCHEMICAL CHARACTERIZATION .1. [J].