IMPROVED HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPH WITH 3 MULTILAYER COILS CONNECTED IN SERIES .2. SEPARATION OF VARIOUS BIOLOGICAL SAMPLES WITH A SEMIPREPARATIVE COLUMN

被引：13

作者：

ITO, Y ^{[1
]}

OKA, H ^{[1
]}

LEE, YW ^{[1
]}

机构：

[1] RES TRIANGLE INST,RES TRIANGLE PK,NC 27709

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 498卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(01)84245-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The semipreparative capability of the newly developed high-speed counter-current chromatograph equipped with a set of three multilayer coils has been demonstrated in separations of a variety of biological samples including triterpenoic acids, indole auxins, bacitracin, flavonoids and tetracycline derivatives, each with a suitable two-phase solvent system. The sample quantities ranging from 50 to 500 mg were efficiently separated within a few hours. The separation of tetracycline derivatives was remarkably improved by adding ammonium acetate to the solvent system. © 1990.

引用

页码：169 / 178

页数：10

共 4 条

[1] HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY [J].