Isolation of cDNAs Encoding Asparagine Synthetase from Coronilla rostrata

被引:0
作者
Artanti, Nina [1 ]
McFarlane, Ian James [2 ]
机构
[1] Indonesian Inst Sci LIPI, Res Ctr Chem, Bandung, Indonesia
[2] Univ New South Wales, Sch Biochem & Mol Genet, Sydney, NSW, Australia
来源
ANNALES BOGORIENSES-JOURNAL OF TROPICAL GENERAL BOTANY | 2012年 / 16卷 / 01期
关键词
cDNA; nitrogen metabolism; asparagine synthetase; Coronilla rostrata;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Asparagine synthetase (AS) is one of the enzyme involves in ammonium metabolism in plant, it catalyzes the transfer of the amino group of glutamine to aspartate giving asparagine. Asparagine serves as a major nitrogen transport and storage molecule in many higher plants. This research used legume plant Coronilla rostrata callus culture for primary and secondary metabolism study. On primary metabolism, the culture were used to study the phenomenon of asparagine accumulation. Because of the responsiveness of the culture, we investigated the use of this culture for model system to conduct molecular biology study of the nitrogen metabolism. The aim of this study was to isolate... cDNAs enconding Asparagine synthetase from C. rostrata because of the asparagine accumulate in this culture. Several PCR based approaches were conducted such as RT-PCR, LM-PCR and RACE. Using these methods, two AS cDNAs was isolated from C. rostrata. AS1 (GenBank no AY081945) which a complete cDNA sequence and AS2 which a partial cDNA sequence. (GenBank no AF488726). These two cDNAs had high homology with the legume AS.
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页码:9 / 18
页数:10
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