Colorimetric method for simultaneous estimation of amlodipine besylate from plasma

Aim: The present work was to develop the method of analysis which can estimate drug in combined form without prior separation. Materials and method: By using UV spectroscopy colorimetric method was used for determination of Amlodipine besylate (AML) from plasma. Result and conclusion: This method is based on the formation of green colour in reaction between AML and 0.4 % Ferric chloride (FC) and 0.2 % Potassium ferricyanide (PF). The absorbance was measured at 775 nm. Result of tablet analysis showed % S.D. values in the range of 098.22 to 100,63%. Standard deviation value for tablet analysis by using methanol ranging from 98.01 to 101,13 % which proves the ability of the method to remain unaffected by small but deliberate change in reaction conditions and this method is used for estimation of AML from biological samples.