SITE-DIRECTED MUTAGENESIS IN THE ACTIVE-SITE OF THE HERPES-SIMPLEX VIRUS TYPE-1 THYMIDINE KINASE GENE

被引：8

作者：

FETZER, J ^{[1
]}

FOLKERS, G ^{[1
]}

MULLER, I ^{[1
]}

KEIL, GM ^{[1
]}

机构：

[1] FED RES CTR VIRUS DIS ANIM, TUBINGEN, GERMANY

来源：

VIRUS GENES | 1993年 / 7卷 / 02期

关键词：

HSV-1 THYMIDINE KINASE; SITE-DIRECTED MUTAGENESIS; HSV-1 TK ANTISERUM;

D O I：

10.1007/BF01702400

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The thymidine kinase (TK) of herpes simplex virus type 1 (HSV-1) contains three regions of homology to other ATP utilizing enzymes. We have altered one region of the protein, which seems to play an important role in phosphorylating substrates by site-directed mutagenesis. When the aspartate 162 was changed to asparagine, the enzyme lost its activity. To identify the inactive protein, expressed by a vaccinia vector in eukaryotic cells, a monospecific antiserum against a bacterial tryptophan E-HSV-1 TK fusion protein was made. These results support the suggestion that aspartate 162 is essential for the enzymatic activity.

引用

页码：205 / 209

页数：5

共 50 条

[31] Site-directed mutagenesis of the essential arginine of the formate dehydrogenase active centre [J].

Galkin, AG ;

Kutsenko, AS ;

Bajulina, NP ;

Esipova, NG ;

Lamzin, VS ;

Mesentsev, AV ;

Shelukho, DV ;

Tikhonova, TV ;

Tishkov, VI ;

Ustinnikova, TB ;

Popov, VO .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2002, 1594 (01) :136-149

[32] Site-directed mutagenesis of key amino acids in the active site of amylosucrase from Neisseria polysaccharea [J].

Albenne, C ;

De Montalk, GP ;

Monsan, P ;

Skov, L ;

Mirza, O ;

Gajhede, M ;

Remaud-Simeon, M .

BIOLOGIA, 2002, 57 :119-128

[33] Site-directed mutagenesis of long QT syndrome KCNQ1 gene in vitro [J].

Li W. ;

Yang J. ;

Du R. ;

Tian L. ;

Wang B. ;

Xu Q. ;

Ke Q. ;

Wang Q. .

Frontiers of Medicine in China, 2008, 2 (1) :100-104

[34] Fluorescence and site-directed mutagenesis studies of interleukin 1 beta [J].

Epps, DE ;

Yem, AW ;

McGee, JM ;

Tomich, CSC ;

Curry, KA ;

Chosay, JG ;

Deibel, MR .

CYTOKINE, 1997, 9 (03) :149-156

[35] ALTERATION OF THE AMINO-ACID SUBSTRATE-SPECIFICITY OF CLOSTRIDIAL GLUTAMATE-DEHYDROGENASE BY SITE-DIRECTED MUTAGENESIS OF AN ACTIVE-SITE LYSINE RESIDUE [J].

WANG, XG ;

BRITTON, KL ;

BAKER, PJ ;

MARTIN, S ;

RICE, DW ;

ENGEL, PC .

PROTEIN ENGINEERING, 1995, 8 (02) :147-152

[36] Site-directed mutagenesis of proline 204 in the 'hinge' region of yeast phosphoglycerate kinase [J].

McHarg, J ;

Kelly, SM ;

Price, NC ;

Cooper, A ;

Littlechild, JA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 259 (03) :939-945

[37] Evaluation by site-directed mutagenesis of active site amino acid residues of Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase [J].

Jabalquinto, AM ;

Laivenieks, M ;

González-Nilo, FD ;

Yévenes, A ;

Encinas, MV ;

Zeikus, JG ;

Cardemil, E .

JOURNAL OF PROTEIN CHEMISTRY, 2002, 21 (06) :393-400

[38] Evaluation by Site-Directed Mutagenesis of Active Site Amino Acid Residues of Anaerobiospirillum succiniciproducens Phosphoenolpyruvate Carboxykinase [J].

Ana María Jabalquinto ;

Maris Laivenieks ;

Fernando D. González-Nilo ;

Alejandro Yévenes ;

María Victoria Encinas ;

J. Gregory Zeikus ;

Emilio Cardemil .

Journal of Protein Chemistry, 2002, 21 :393-400

[39] Homology modeling of the structure of tobacco acetohydroxy acid synthase and examination of the active site by site-directed mutagenesis [J].

Le, DT ;

Yoon, MY ;

Kim, YT ;

Choi, JD .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 317 (03) :930-938

[40] Site-directed mutagenesis and structural interpretation of the nidogen binding site of the laminin gamma 1 chain [J].

Poschl, E ;

Mayer, U ;

Stetefeld, J ;

Baumgartner, R ;

Holak, TA ;

Huber, R ;

Timpl, R .

EMBO JOURNAL, 1996, 15 (19) :5154-5159

← 1 2 3 4 5 →