INVITRO EFFECTS OF HYALURONAN ON PROSTAGLANDIN-E2 INDUCTION BY INTERLEUKIN-1 IN RABBIT ARTICULAR CHONDROCYTES

被引：36
作者：
AKATSUKA, M
YAMAMOTO, Y
TOBETTO, K
YASUI, T
ANDO, T
机构：
[1] Division of Biochemical Pharmacology, Research Laboratories, Maruho Co., Ltd., Osaka, 531, 1-8-23 Oyodo Naka, Kita-ku
来源：
AGENTS AND ACTIONS | 1993年 / 38卷 / 1-2期
关键词：
D O I：
10.1007/BF02027223
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Effects of hyaluronan (HA) on the prostaglandin E2 (PGE2) production induced by recombinant human interleukin-1beta (rhIL-1beta) in rabbit articular chondrocytes were studied in vitro. The rhIL-1beta-induced PGE2 production was dose-dependently inhibited by HA. HA with the highest molecular weight (M(r) = 2.0 x 10(6)) exhibited an inhibitory effect that was statistically more significant than the same polymer of lower molecular weights (M(r) = 1.0 x 10(6), 0. 5 x 10(6)). This effect was observed in both young and adult rabbit articular chondrocytes. Since PGE2 has been implicated as a mediator of inflammatory joint diseases, our observations suggest that HA may elicit an anti-inflammatory effect by inhibiting PGE2 production.
引用
收藏
页码：122 / 125
页数：4
相关论文
共 25 条
[1]   EFFECTS OF SODIUM HYALURONATE ON THE NOCICEPTIVE RESPONSE OF RATS WITH EXPERIMENTALLY INDUCED ARTHRITIS [J].
AIHARA, S ;
MURAKAMI, N ;
ISHII, R ;
KARIYA, K ;
AZUMA, Y ;
HAMADA, K ;
UMEMOTO, J ;
MAEDA, S .
FOLIA PHARMACOLOGICA JAPONICA, 1992, 100 (04) :359-365
[2]  
CHANG J, 1986, J IMMUNOL, V136, P1283
[3]  
CORTIVO R, 1990, CELL BIOL INT REP, V14, P111
[4]   INTERLEUKIN-2-INDEPENDENT STIMULATION OF RABBIT CHONDROCYTE COLLAGENASE AND PROSTAGLANDIN-E2 PRODUCTION BY AN INTERLEUKIN 1-LIKE FACTOR [J].
EVEQUOZ, V ;
BETTENS, F ;
KRISTENSEN, F ;
TRECHSEL, U ;
STADLER, BM ;
DAVER, JM ;
DEWECK, AL ;
FLEISCH, H .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1984, 14 (06) :490-495
[5]  
FORRESTER JV, 1981, J CELL SCI, V48, P315
[6]  
FULKERSON JP, 1983, CLIN ORTHOP RELAT R, V179, P266
[7]  
HASCALL VC, 1974, J BIOL CHEM, V249, P4232
[8]   HYALURONIC-ACID IN HUMAN ARTICULAR-CARTILAGE - AGE-RELATED-CHANGES IN CONTENT AND SIZE [J].
HOLMES, MWA ;
BAYLISS, MT ;
MUIR, H .
BIOCHEMICAL JOURNAL, 1988, 250 (02) :435-441
[9]   EFFECTS OF RECOMBINANT INTERLEUKIN-1-BETA ON PHOSPHOLIPASE-A2 ACTIVITY, PHOSPHOLIPASE-A2 MESSENGER-RNA LEVELS, AND EICOSANOID FORMATION IN RABBIT CHONDROCYTES [J].
KERR, JS ;
STEVENS, TM ;
DAVIS, GL ;
MCLAUGHLIN, JA ;
HARRIS, RR .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (03) :1079-1084
[10]   PROSTAGLANDINS AND ARTICULAR-CARTILAGE METABOLISM - DOES PROSTAGLANDIN PERTURBATION PERPETUATE CARTILAGE DESTRUCTION [J].
LIPPIELLO, L .
SEMINARS IN ARTHRITIS AND RHEUMATISM, 1981, 11 (01) :87-88
123