CLONING AND FUNCTIONAL-CHARACTERIZATION OF A COCAINE-SENSITIVE DOPAMINE TRANSPORTER

被引：288

作者：

GIROS, B

ELMESTIKAWY, S

BERTRAND, L

CARON, MG

机构：

[1] DUKE UNIV, MED CTR, HOWARD HUGHES MED INST LABS, DURHAM, NC 27710 USA

[2] DUKE UNIV, MED CTR, DEPT MED, DURHAM, NC 27710 USA

来源：

FEBS LETTERS | 1991年 / 295卷 / 1-3期

关键词：

DOPAMINE TRANSPORTER; TRANSMEMBRANE DOMAIN; SUBSTANTIA-NIGRA PARS COMPACTA; VENTRAL TEGMENTAL;

D O I：

10.1016/0014-5793(91)81406-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the cloning of a rat cDNA encoding a functional dopamine transporter. This cDNA, derived from an intron-containing gene, encodes a protein of 620 amino acids. Hydropathicity analysis of the protein sequence suggests the presence of 12 putative transmembrane domains. The protein displays considerable identity with transporters for noradrenaline and GABA (64 and 30%, respectively). Transient expression of the cDNA in COS7 cells directs the expression of dopamine uptake activity with appropriate pharmacology and in a sodium-dependent fashion. In situ hybridization reveals that the mRNA for this transporter is expressed in the substantia nigra and ventral tegmental area, regions that contain dopaminergic cell bodies.

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页码：149 / 154

页数：6

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