TRANSFORMATION OF HUMAN BREAST EPITHELIAL-CELLS BY C-HA-RAS ONCOGENE

被引:158
作者
BASOLO, F
ELLIOTT, J
TAIT, L
CHEN, XQ
MALONEY, T
RUSSO, IH
PAULEY, R
MOMIKI, S
CAAMANO, J
KLEINSZANTO, AJP
KOSZALKA, M
RUSSO, J
机构
[1] MICHIGAN CANC FDN, DEPT PATHOL, 110 E WARREN AVE, DETROIT, MI 48201 USA
[2] FOX CHASE CANC INST, EXPTL HISTOPATHOL SERV, PHILADELPHIA, PA 19111 USA
关键词
CELL TRANSFORMATION; HUMAN; BREAST; C-HA-RAS; ONCOGENE;
D O I
10.1002/mc.2940040106
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To determine whether the c-Ha-ras oncogene plays a role in the initiation of mammary carcinogenesis, an immortalized human breast epithelial cell line, MCF-10A, was transfected with the plasmid vector pHo6T1 containing the T24 Ha-ras oncogene and the aminoglycoside phosphotransferase gene, which confers resistance to geneticin. Transfected cells exhibited an altered pattern of growth and tridimensional morphology in collagen gel. They also exhibited anchorage-independent growth and loss of requirement for hormones and epidermal growth factor; in addition, they expressed invasiveness and increased collagenolytic activity in an in vitro system and became tumorigenic in irradiated nude mice, all properties indicative of malignant transformation. Transformed cells contained the mutated c-Ha-ras oncogene and expressed the p21 mutated protein. These data indicate that the c-Ha-ras oncogene is capable of inducing malignant phenotypes in immortalized human breast epithelial cells.
引用
收藏
页码:25 / 35
页数:11
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