DETERMINATION OF VITAMIN-K-1 IN CANOLA-OILS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH MENAQUINONE-4 AS AN INTERNAL STANDARD

被引：18

作者：

GAO, ZH ^{[1
]}

ACKMAN, RG ^{[1
]}

机构：

[1] TECH UNIV NOVA SCOTIA,DEPT FOOD SCI & TECHNOL,HALIFAX,NS B3J 2X4,CANADA

来源：

FOOD RESEARCH INTERNATIONAL | 1995年 / 28卷 / 01期

关键词：

CANOLA OIL; VITAMIN-K-1; MENAQUINONE-4; VITAMIN ANALYSIS; ENZYMATIC HYDROLYSIS; HPLC; POSTCOLUMN REDUCTION;

D O I：

10.1016/0963-9969(95)93332-O

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

An effective high performance liquid chromatography (HPLC) method for measuring vitamin K-1 in canola oil with menaquinone-4 (MK-4) as an internal standard is described. The reliability of this method was examined in detail. No endogenous MK-4 or K vitamins other than vitamin K-1 were found in canola oil. The oil-soluble vitamin fraction was concentrated by a preliminary enzymatic hydrolysis of triacylglycerides, further purified by a silica SPE cartridge, and then analyzed by reverse-phase HPLC with fluorescence detection after post-column chemical reduction with a zinc dust column. Vitamin K-1 in canola oil was confirmed to occur at the level of nearly 400 mu g/100 g of oil, six samples giving a range between 348 mu g/100 g for a crude oil and 125 mu g/100 g for one commercial retail oil. The exact level of Vitamin K-1 in any one oil might be related to the oil origin, refining, and retail display conditions.

引用

页码：61 / 69

页数：9

共 24 条

[1]

Ackman R. G, 1990, CANOLA RAPESEED PROD, DOI [10.1007/978-1-4615-3912-4_6, DOI 10.1007/978-1-4615-3912-4_6]

[2]

ACKMAN RG, 1983, HIGH LOW ERUCIC ACID, P84

[3]

BALL GFM, 1988, FAT SOLUBLE VITAMIN, P52

[4] EVALUATION OF AN HPLC METHOD FOR THE DETERMINATION OF PHYLLOQUINONE (VITAMIN-K-1) IN VARIOUS FOOD MATRICES [J].

BOOTH, SL ;

DAVIDSON, KW ;

SADOWSKI, JA .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1994, 42 (02) :295-300

[5]

BUDAVARI S, 1989, MERCK INDEX, P868

[6]

BUENO MP, 1983, J ASSOC OFF ANA CHEM, V66, P1063

[7]

CHATFIELD C, 1983, STAT TECHNOLOGY COUR, P237

[8]

Dunphy P.J., 1971, METHODS ENZYMOLOGY, VVolume 18, P407, DOI [10.1016/S0076-6879(71)18035-4, DOI 10.1016/S0076-6879(71)18035-4]

[9] VITAMIN-K1 (PHYLLOQUINONE) CONTENT OF EDIBLE OILS - EFFECTS OF HEATING AND LIGHT EXPOSURE [J].

FERLAND, G ;

SADOWSKI, JA .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1992, 40 (10) :1869-1873

[10]

FERLAND G, 1989, FASEB Journal, V3, pA662

← 1 2 3 →