HIGH EXPRESSION OF HUMAN CYTOMEGALOVIRUS (HCMV)-GB PROTEIN IN CELLS INFECTED WITH A VACCINIA-GB RECOMBINANT - THE IMPORTANCE OF THE GB PROTEIN IN HCMV IMMUNITY

被引:39
作者
GONCZOL, E
DETAISNE, C
HIRKA, G
BERENCSI, K
LIN, WC
PAOLETTI, E
PLOTKIN, S
机构
[1] VIROGENET,TROY,NY 12180
[2] CHILDRENS HOSP,PHILADELPHIA,PA 19104
关键词
HCMV; GB PROTEIN; VACCINIA RECOMBINANT; HCMV NEUTRALIZING ANTIBODIES;
D O I
10.1016/0264-410X(91)90187-B
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The human cytomegalovirus (HCMV), Towne strain, glycoprotein B (gB) gene was cloned into a vaccinia vector (Copenhagen strain) under the control of the H6 early and late vaccinia promoters (Vac-gB recombinant). The gB protein was expressed in a high percentage of the Vac-gB-infected cells throughout the virus replication cycle. Cytosine-arabinoside (ara-C) did not influence the expression of the gB protein early after infection (5 h), but did inhibit it later in viral replication (7-29 h). The Vac-gB recombinant induced HCMV neutralizing antibodies in guinea-pigs. Cells infected with the Vac-gB recombinant absorbed 50-88% of neutralizing activity of human sera obtained from volunteers previously inoculated with the Towne or Toledo strains and from naturally seropositive individuals.
引用
收藏
页码:631 / 637
页数:7
相关论文
共 45 条
[1]   CELL-SURFACE ANTIGENS OF A CLONAL HUMAN EMBRYONAL CARCINOMA CELL-LINE - MORPHOLOGICAL AND ANTIGENIC DIFFERENTIATION IN CULTURE [J].
ANDREWS, PW ;
GOODFELLOW, PN ;
SHEVINSKY, LH ;
BRONSON, DL ;
KNOWLES, BB .
INTERNATIONAL JOURNAL OF CANCER, 1982, 29 (05) :523-531
[2]   HUMAN CYTOMEGALOVIRUS-SPECIFIC CYTO-TOXIC T-CELLS - RELATIVE FREQUENCY OF STAGE-SPECIFIC CTL RECOGNIZING THE 72-KD IMMEDIATE EARLY PROTEIN AND GLYCOPROTEIN-B EXPRESSED BY RECOMBINANT VACCINIA VIRUSES [J].
BORYSIEWICZ, LK ;
HICKLING, JK ;
GRAHAM, S ;
SINCLAIR, J ;
CRANAGE, MP ;
SMITH, GL ;
SISSONS, JGP .
JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 168 (03) :919-931
[3]   INDUCTION OF COMPLEMENT-DEPENDENT AND COMPLEMENT-INDEPENDENT NEUTRALIZING ANTIBODIES BY RECOMBINANT-DERIVED HUMAN CYTOMEGALO-VIRUS GP55-116 (GB) [J].
BRITT, WJ ;
VUGLER, L ;
STEPHENS, EB .
JOURNAL OF VIROLOGY, 1988, 62 (09) :3309-3318
[4]   CELL-SURFACE EXPRESSION OF HUMAN CYTOMEGALOVIRUS (HCMV) GP55-116 (GB) - USE OF HCMV-RECOMBINANT VACCINIA VIRUS-INFECTED CELLS IN ANALYSIS OF THE HUMAN NEUTRALIZING ANTIBODY-RESPONSE [J].
BRITT, WJ ;
VUGLER, L ;
BUTFILOSKI, EJ ;
STEPHENS, EB .
JOURNAL OF VIROLOGY, 1990, 64 (03) :1079-1085
[6]  
BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5
[7]   TEMPORAL REGULATION OF INFLUENZA HEMAGGLUTININ EXPRESSION IN VACCINIA VIRUS RECOMBINANTS AND EFFECTS ON THE IMMUNE-RESPONSE [J].
COUPAR, BEH ;
ANDREW, ME ;
BOTH, GW ;
BOYLE, DB .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1986, 16 (12) :1479-1487
[8]   IDENTIFICATION AND EXPRESSION OF A HUMAN CYTOMEGALO-VIRUS GLYCOPROTEIN WITH HOMOLOGY TO THE EPSTEIN-BARR VIRUS BXLF2 PRODUCT, VARICELLA-ZOSTER VIRUS-GPIII, AND HERPES-SIMPLEX VIRUS TYPE-1 GLYCOPROTEIN-H [J].
CRANAGE, MP ;
SMITH, GL ;
BELL, SE ;
HART, H ;
BROWN, C ;
BANKIER, AT ;
TOMLINSON, P ;
BARRELL, BG ;
MINSON, TC .
JOURNAL OF VIROLOGY, 1988, 62 (04) :1416-1422
[9]   IDENTIFICATION OF THE HUMAN CYTOMEGALOVIRUS GLYCOPROTEIN-B GENE AND INDUCTION OF NEUTRALIZING ANTIBODIES VIA ITS EXPRESSION IN RECOMBINANT VACCINIA VIRUS [J].
CRANAGE, MP ;
KOUZARIDES, T ;
BANKIER, AT ;
SATCHWELL, S ;
WESTON, K ;
TOMLINSON, P ;
BARRELL, B ;
HART, H ;
BELL, SE ;
MINSON, AC ;
SMITH, GL .
EMBO JOURNAL, 1986, 5 (11) :3057-3063
[10]   ANTIBODY-RESPONSE TO CYTOMEGALO-VIRUS POLYPEPTIDES CAPTURED BY MONOCLONAL-ANTIBODIES ON THE SOLID-PHASE IN ENZYME IMMUNOASSAYS [J].
CREMER, NE ;
COSSEN, CK ;
SHELL, GR ;
PEREIRA, L .
JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 21 (04) :517-521