HIGH-RESOLUTION MAPPING OF HUMAN CHROMOSOME-11 BY INSITU HYBRIDIZATION WITH COSMID CLONES

被引：1441
作者：
LICHTER, P
TANG, CJC
CALL, K
HERMANSON, G
EVANS, GA
HOUSMAN, D
WARD, DC
机构：
[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139
[2] SALK INST BIOL STUDIES,MOLEC GENET LAB,SAN DIEGO,CA 92138
来源：
SCIENCE | 1990年 / 247卷 / 4938期
关键词：
D O I：
10.1126/science.2294592
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Cosmid clones containing human DNA inserts have been mapped on chromosome 11 by fluorescence in situ hybridization under conditions that suppress signal from repetitive DNA sequences. Thirteen known genes, one chromosome 11-specific DNA repeat, and 36 random clones were analyzed. High-resolution mapping was facilitated by using digital imaging microscopy and by analyzing extended (prometaphase) chromosomes. The map coordinates established by in situ hybridization showed a one to one correspondence with those determined by Southern (DNA) blot analysis of hybrid cell lines containing fragments of chromosome 11. Furthermore, by hybridizing three or more cosmids simultaneously, gene order on the chromosome could be established unequivocaily. These results demonstrate the feasibility of rapidly producing high-resolution maps of human chromosomes by in situ hybridization.
引用
收藏
页码：64 / 69
页数：6
相关论文
共 54 条
[1]   SENSITIVE AND HIGH-RESOLUTION INSITU HYBRIDIZATION TO HUMAN-CHROMOSOMES USING BIOTIN LABELED PROBES - ASSIGNMENT OF THE HUMAN THYMOCYTE CD1 ANTIGEN GENES TO CHROMOSOME-1 [J].
ALBERTSON, DG ;
FISHPOOL, R ;
SHERRINGTON, P ;
NACHEVA, E ;
MILSTEIN, C .
EMBO JOURNAL, 1988, 7 (09) :2801-2805
[2]   ISOLATION OF THE HUMAN INSULIN-LIKE GROWTH-FACTOR GENES - INSULIN-LIKE GROWTH FACTOR-II AND INSULIN GENES ARE CONTIGUOUS [J].
BELL, GI ;
GERHARD, DS ;
FONG, NM ;
SANCHEZPESCADOR, R ;
RALL, LB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (19) :6450-6454
[3]   DIRECT VISUALIZATION OF SINGLE COPY GENES ON BANDED METAPHASE CHROMOSOMES BY NONISOTOPIC INSITU HYBRIDIZATION [J].
BHATT, B ;
BURNS, J ;
FLANNERY, D ;
MCGEE, JO .
NUCLEIC ACIDS RESEARCH, 1988, 16 (09) :3951-3961
[4]   DETECTION OF VIRAL GENOMES IN CULTURED-CELLS AND PARAFFIN-EMBEDDED TISSUE-SECTIONS USING BIOTIN-LABELED HYBRIDIZATION PROBES [J].
BRIGATI, DJ ;
MYERSON, D ;
LEARY, JJ ;
SPALHOLZ, B ;
TRAVIS, SZ ;
FONG, CKY ;
HSIUNG, GD ;
WARD, DC .
VIROLOGY, 1983, 126 (01) :32-50
[5]  
BUDARF M, 1989, AM J HUM GENET, V45, P128
[6]   GENOME INSTABILITY IN A REGION OF HUMAN DNA ENRICHED IN ALU REPEAT SEQUENCES [J].
CALABRETTA, B ;
ROBBERSON, DL ;
BARRERASALDANA, HA ;
LAMBROU, TP ;
SAUNDERS, GF .
NATURE, 1982, 296 (5854) :219-225
[7]   GENETIC-ORIGIN OF MUTATIONS PREDISPOSING TO RETINOBLASTOMA [J].
CAVENEE, WK ;
HANSEN, MF ;
NORDENSKJOLD, M ;
KOCK, E ;
MAUMENEE, I ;
SQUIRE, JA ;
PHILLIPS, RA ;
GALLIE, BL .
SCIENCE, 1985, 228 (4698) :501-503
[8]   DETECTION OF SINGLE-COPY GENES BY NONISOTOPIC INSITU HYBRIDIZATION ON HUMAN-CHROMOSOMES [J].
CHERIF, D ;
BERNARD, O ;
BERGER, R .
HUMAN GENETICS, 1989, 81 (04) :358-362
[9]   THE MOLECULAR GENETICS OF HUMAN-HEMOGLOBIN [J].
COLLINS, FS ;
WEISSMAN, SM .
PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, 1984, 31 :315-462
[10]   SEGREGATION OF THE HUNTINGTON DISEASE REGION OF HUMAN-CHROMOSOME 4 IN A SOMATIC-CELL HYBRID [J].
COX, DR ;
PRITCHARD, CA ;
UGLUM, E ;
CASHER, D ;
KOBORI, J ;
MYERS, RM .
GENOMICS, 1989, 4 (03) :397-407
123456