NERVE GROWTH-FACTOR INDUCES 5-HT3 RECOGNITION SITES IN RAT PHEOCHROMOCYTOMA (PC12) CELLS

被引：11

作者：

GORDON, JC ^{[1
]}

ROWLAND, HC ^{[1
]}

机构：

[1] AH ROBINS RES LABS,DEPT MOLEC BIOL,RICHMOND,VA 23261

来源：

LIFE SCIENCES | 1990年 / 46卷 / 20期

关键词：

D O I：

10.1016/0024-3205(90)90459-5

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

In rat pheochromocytoma (PC12) cells, nerve growth factor (7S NGF) induced the expression of recognition sites that bind the specific 5-HT3 antagonist (S-) [3H] zacopride. Culturing PC12 cells for 8-12 days in the presence of 50 ng/ml NGF increased the density (Bmax) of (S-) [3H] zacopride binding sites in cell membranes (0-100, 000 x g fraction) from 0 to 105 fmoles/mg protein. This binding exhibited high affinity for (S-) [3H] zacopride (Kd=0.8 nM), was specific (>95%), and was inhibited by 5-HT3 compounds with a rank of potency (quipazine>ICS 205- 930>GR38032F>BRL 24924≈MDL 72222>phenylbiguanide≥serotonin>2-methyl-serotonin>metoclopramide) which was distinct from neuroblastoma cells. Thus, NGF-differentiated PC12 cells possess a 5-HT3 receptor and should be useful to investigate its regulation and biochemical mechanism of action. © 1990.

引用

页码：1435 / 1442

页数：8

共 42 条

[1] ACTIVATION OF 5-HT3 RECEPTOR SUBTYPES CAUSES RAPID EXCITATION OF RABBIT PARASYMPATHETIC NEURONS [J].