IMMUNOLOGICAL DETECTION OF SINGLE AMINO ACID SUBSTITIONS IN ALKALINE PHOSPHATASE

被引：66
作者：
COCKS, GT
WILSON, AC
机构：
[1] Department of Biochemistry, University of California
来源：
SCIENCE | 1969年 / 164卷 / 3876期
关键词：
D O I：
10.1126/science.164.3876.188
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Antisemms to wild-type alkaline phosphatase from Escherichia coli were prepared and tested for reactivity with phosphatases altered by point mutations. Eight out of the nine mutant enzymes were distinguished from the wild type with quantitative microcomplement fixation. The structural changes are among the smallest yet observed immunologically.
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页码：188 / &
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