DEVELOPMENT OF A MELANIN-BASED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY STATIONARY-PHASE AND ITS USE IN THE STUDY OF DRUG-MELANIN BINDING INTERACTIONS

被引:29
作者
IBRAHIM, H
AUBRY, AF
机构
[1] ROYAL VICTORIA HOSP,DEPT MED,MONTREAL,PQ H3A 1A1,CANADA
[2] MCGILL UNIV,DEPT ONCOL,MONTREAL,PQ,CANADA
关键词
D O I
10.1006/abio.1995.1412
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Drug-melanin interactions were studied using a melanin-based HPLC column. Two approaches were chosen for the preparation of the stationary phases: covalent coupling of synthetic L-dopa melanin and in situ polymerization of L-dopa. Retention of a series of phenothiazines on melanin-based stationary phases was attributed to binding to melanin. Frontal affinity chromatography experiments on one melanin-based column allowed us to calculate the affinity and binding capacity of chlorpromazine and promethazine. A competition was observed between chlorpromazine and haloperidol which was qualitatively consistent with previously described results. Data indicated that the interaction was not a simple competition at one site. (C) 1995 Academic Press, Inc.
引用
收藏
页码:272 / 277
页数:6
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