A NOVEL-APPROACH TO THE LABORATORY DIAGNOSIS OF CHLAMYDIA-TRACHOMATIS INFECTIONS USING MONOCLONAL ANTIIDIOTYPIC ANTIBODIES

被引：12

作者：

LAFERRIERE, C

PEELING, RW

TACKABERRY, ES

HAMEL, J

DILLON, JA

BRODEUR, BR

机构：

[1] LAB CTR DIS CONTROL, NATL LAB IMMUNOL,HPB BLDG 7,RM 181, TUNNEYS PASTURE, OTTAWA K1A 0L2, ONTARIO, CANADA

[2] LAB CTR DIS CONTROL, NATL LAB SEXUALLY TRANSMITTED DIS, OTTAWA K1A 0L2, ONTARIO, CANADA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1993年 / 163卷 / 01期

关键词：

IMMUNOASSAY; IDIOTYPE ANTIIDIOTYPE; HEAT-SHOCK PROTEIN; CHLAMYDIA-TRACHOMATIS;

D O I：

10.1016/0022-1759(93)90246-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a novel enzyme immunoassay (EIA) for the specific detection of Chlamydia trachomatis utilizing a monoclonal anti-idiotypic antibody to an antibody directed against a chlamydia specific epitope on 60 kDa heat-shock protein (HSP60). The basis of the assay is the inhibition of the binding of idiotype to anti-idiotype by antigen present in test samples. Two configurations of the assay were developed: a blocking EIA and a competition EIA. Greater sensitivity was observed using the competition EIA, with the assay detecting purified recombinant HSP60 and purified chlamydia in a concentration-dependent manner from 0,01 to 10 mug protein and from 0.5 to 12 mug total protein, respectively. The assay is highly specific and offers several potential advantages over currently available EIAs for the detection of this pathogen.

引用

页码：123 / 131

页数：9

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