2 PK VALUES OF THE OXIDIZED RIESKE [2FE-2S] CLUSTER OBSERVED BY CD SPECTROSCOPY

被引：55

作者：

LINK, TA

机构：

[1] Universitätsklinikum Frankfurt, ZBC, Therapeutische Biochemie

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1994年 / 1185卷 / 01期

关键词：

PK VALUE; RIESKE CLUSTER; MITOCHONDRIAL-BC(1) COMPLEX; REDOX POTENTIAL; CD SPECTROSCOPY;

D O I：

10.1016/0005-2728(94)90196-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pH dependence of the CD spectrum of the oxidised 'Rieske' [2Fe-2S] cluster was measured over pH = 6.1 to 10.6. The spectral changes could be fitted with two pK values of 7.7 and 9.1. No pH dependent spectral changes were observed on the reduced protein. The far UV CD spectrum of the protein was the same at pH = 6.0 and pH = 10.7; therefore, secondary structure changes are not responsible for the changes in the CD spectrum. It is concluded that the pK values belong to groups directly associated with the [2Fe-2S] cluster, most likely to its histidine ligands.

引用

页码：81 / 84

页数：4

共 15 条

[1] ELECTRON-SPIN ECHO ENVELOPE MODULATION SPECTROSCOPY SUPPORTS THE SUGGESTED COORDINATION OF 2 HISTIDINE LIGANDS TO THE RIESKE FE-S CENTERS OF THE CYTOCHROME-B6F COMPLEX OF SPINACH AND THE CYTOCHROME-BC1 COMPLEXES OF RHODOSPIRILLUM-RUBRUM, RHODOBACTER-SPHAEROIDES R-26, AND BOVINE HEART-MITOCHONDRIA [J].