CONFORMATION OF INTERCALATED DNA PLASMIDS INVESTIGATED BY CIRCULAR-DICHROISM AND DYNAMIC LIGHT-SCATTERING

被引：10

作者：

CHIRICO, G ^{[1
]}

LUNELLI, L ^{[1
]}

BALDINI, G ^{[1
]}

机构：

[1] UNIV MILAN,DIPARTMENTO FIS,VIA CELORIA 16,I-20133 MILAN,ITALY

来源：

BIOPHYSICAL CHEMISTRY | 1990年 / 38卷 / 03期

关键词：

Circular dichroism; Conformation; DNA; Dynamic light scattering; Ethidium bromide; Plasmid;

D O I：

10.1016/0301-4622(90)87002-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two DNA plasmids, pEGF and pACL29, intercalated with ethidium bromide (EB), have been examined by circular dichroism (CD) and dynamic light scattering (DLS). CD and DLS data show significant changes when the EB/DNA (phosphates) ratio reaches a value of r ≅ 0.13. The translational and rotational diffusion coefficients, predicted assuming that plasmids can be described by a string of beads, and the CD spectrum, suggest that a transition from an interwound to a toroidal conformation is likely to occur. © 1990.

引用

页码：201 / 211

页数：11

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