In this paper a review is given about the most important lipase-producing yeasts as well as the yield of lipase production. In order to find out the optimal conditions for lipase production by yeasts, it is necessary to investigate the influence of the main factors affecting lipase synthesis such as carbon and nitrogen sources, activators and inhibitors, interface-affecting agents, temperature, pH and inoculum. Besides the yield of lipase as an efficiency factor of fermentation and the substrate specificity of lipase, playing an important role in applications, are described.