TRANSCRIPTIONAL REPRESSION MEDIATED BY THE WT1 WILMS-TUMOR GENE-PRODUCT

被引：494
作者：
MADDEN, SL
COOK, DM
MORRIS, JF
GASHLER, A
SUKHATME, VP
RAUSCHER, FJ
机构：
[1] WISTAR INST, 3601 SPRUCE ST, PHILADELPHIA, PA 19104 USA
[2] HOWARD HUGHES MED INST, DEPT MED MOLEC GENET & CELL BIOL, CHICAGO, IL 60637 USA
来源：
SCIENCE | 1991年 / 253卷 / 5027期
关键词：
D O I：
10.1126/science.1654597
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The wt1 gene, a putative tumor suppressor gene located at the Wilms tumor (WT) locus on chromosome 11p13, encodes a zinc finger-containing protein that binds to the same DNA sequence as EGR-1, a mitogen-inducible immediate-early gene product that activates transcription. The transcriptional regulatory potential of WT1 has not been demonstrated. In transient transfection assays, the WT1 protein functioned as a repressor of transcription when bound to the EGR-1 site. The repression function was mapped to the glutamine- and proline-rich NH2-terminus of WT1; fusion of this domain to the zinc finger region of EGR-1 converted EGR-1 into a transcriptional repressor.
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页码：1550 / 1553
页数：4
相关论文
共 31 条
[1]  
ANDERSSON S, 1989, J BIOL CHEM, V264, P8222
[2]   ISOLATION, CHARACTERIZATION, AND EXPRESSION OF THE MURINE WILMS-TUMOR GENE (WT1) DURING KIDNEY DEVELOPMENT [J].
BUCKLER, AJ ;
PELLETIER, J ;
HABER, DA ;
GLASER, T ;
HOUSMAN, DE .
MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (03) :1707-1712
[3]   ISOLATION AND CHARACTERIZATION OF A ZINC FINGER POLYPEPTIDE GENE AT THE HUMAN CHROMOSOME-11 WILMS TUMOR LOCUS [J].
CALL, KM ;
GLASER, T ;
ITO, CY ;
BUCKLER, AJ ;
PELLETIER, J ;
HABER, DA ;
ROSE, EA ;
KRAL, A ;
YEGER, H ;
LEWIS, WH ;
JONES, C ;
HOUSMAN, DE .
CELL, 1990, 60 (03) :509-520
[4]  
COOK DM, UNPUB
[5]   ANALYSIS OF SP1 INVIVO REVEALS MULTIPLE TRANSCRIPTIONAL DOMAINS, INCLUDING A NOVEL GLUTAMINE-RICH ACTIVATION MOTIF [J].
COUREY, AJ ;
TJIAN, R .
CELL, 1988, 55 (05) :887-898
[6]  
COWELL JK, 1991, ONCOGENE, V6, P595
[7]   PRESENCE OF A POTENT TRANSCRIPTION ACTIVATING SEQUENCE IN THE P53 PROTEIN [J].
FIELDS, S ;
JANG, SK .
SCIENCE, 1990, 249 (4972) :1046-1049
[8]   HOMOZYGOUS DELETION IN WILMS-TUMORS OF A ZINC-FINGER GENE IDENTIFIED BY CHROMOSOME JUMPING [J].
GESSLER, M ;
POUSTKA, A ;
CAVENEE, W ;
NEVE, RL ;
ORKIN, SH ;
BRUNS, GAP .
NATURE, 1990, 343 (6260) :774-778
[9]   TRANSCRIPTIONAL ACTIVATION AND REPRESSION BY FOS ARE INDEPENDENT FUNCTIONS - THE C-TERMINUS REPRESSES IMMEDIATE-EARLY GENE-EXPRESSION VIA CARG ELEMENTS [J].
GIUS, D ;
CAO, XM ;
RAUSCHER, FJ ;
COHEN, DR ;
CURRAN, T ;
SUKHATME, VP .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (08) :4243-4255
[10]   RECOMBINANT GENOMES WHICH EXPRESS CHLORAMPHENICOL ACETYLTRANSFERASE IN MAMMALIAN-CELLS [J].
GORMAN, CM ;
MOFFAT, LF ;
HOWARD, BH .
MOLECULAR AND CELLULAR BIOLOGY, 1982, 2 (09) :1044-1051
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