FLOW CYTOMETRIC DNA CONTENT-ANALYSIS IN PRIMARY LUNG-CANCER - COMPARISON OF RESULTS FROM FRESH AND PARAFFIN-EMBEDDED SPECIMENS

被引：13
|
作者：
ISOBE, H
MIYAMOTO, H
INOUE, K
SHIMIZU, M
ENDO, T
MIZUNO, S
KAWAKAMI, Y
机构：
[1] HOKKAIDO UNIV HOSP,DEPT PATHOL,SAPPORO,JAPAN
[2] HOKKAIDO UNIV,HOSP DENT,CENT CLIN LAB,SAPPORO,HOKKAIDO 060,JAPAN
来源：
JOURNAL OF SURGICAL ONCOLOGY | 1990年 / 43卷 / 01期
关键词：
DNA index; multiple specimens; paraffin‐embedded specimen;
D O I：
10.1002/jso.2930430110
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Tumor DNA content was determined by flow cytometry in 24 surgically resected specimens from patients with primary lung cancer to compare results from fresh and paraffin‐embedded specimens. We found a good correlation in DNA indices between fresh and paraffin‐embedded specimens (r =.978), although one tumor with DNA near‐diploidy was mistaken as DNA diploid in paraffin‐embedded specimens. In the measurements of paraffin‐embedded specimens, eight of 24 tumors were DNA multiploid and showed DNA content heterogeneity. These results indicate that DNA content analysis of paraffin‐embedded specimens is helpful because it makes it easy to analyze multiple specimens from different parts of the tumor. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company
引用
收藏
页码：36 / 39
页数：4
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