QUANTITATION OF GAMMA-TRACE IN HUMAN BIOLOGICAL-FLUIDS - INDICATIONS FOR PRODUCTION IN THE CENTRAL NERVOUS-SYSTEM

被引:197
作者
LOFBERG, H [1 ]
GRUBB, AO [1 ]
机构
[1] UNIV LUND,MALMO GEN HOSP,DEPT DERMATOL,S-21401 MALMO,SWEDEN
关键词
Cerebrospinal fluid proteins; Immunoenzyme techniques; Proteinuria;
D O I
10.3109/00365517909108866
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
γTrace was purified in large amounts from urine and used for the production of a specific rabbit antiserum. An enzyme immunoassay for quantitation of γtrace was developed using the pure protein as a primary standard. Its sensitivity was approximately 30 μg/l. An enzyme amplified single radial immunodiffusion was developed as well. Its sensitivity was approximately 0.3 mg/1. These assays allowed quantitation of γtrace in normal human biological fluids. The following results were obtained (mean ± SD): cerebrospinal fluid: 5.8 ± 2.2 mg/1, plasma: 1.1 ± 0.42 mg/1, saliva: 1.8 ± 0.88 mg/1 and urine: 0.095 ± 0.057 mg/1. Plasma samples from patients with advanced renal failure revealed γtrace values up to 13 times the normal mean plasma value. The results indicate a production of γtrace in the central nervous system and that the protein is primarily catabolized by the kidney. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
引用
收藏
页码:619 / 626
页数:8
相关论文
共 28 条
[1]   COUPLING OF ENZYMES TO PROTEINS WITH GLUTARALDEHYDE . USE OF CONJUGATES FOR DETECTION OF ANTIGENS AND ANTIBODIES [J].
AVRAMEAS, S .
IMMUNOCHEMISTRY, 1969, 6 (01) :43-+
[2]  
BELANGER L, 1976, CLIN CHEM, V22, P198
[3]   OCCURRENCE OF POST-GAMMA PROTEIN IN URINE - A NEW PROTEIN ABNORMALITY [J].
BUTLER, EA ;
FLYNN, FV .
JOURNAL OF CLINICAL PATHOLOGY, 1961, 14 (02) :172-&
[4]  
CEJKA J, 1973, ARCH BIOCHEM BIOPHYS, V157, P168
[5]  
CLAUSEN J, 1961, P SOC EXP BIOL MED, V107, P170
[6]   OCCURRENCE OF BETA-2-MICROGLOBULIN AND POST-GAMMA GLOBULIN IN HUMAN SEMEN [J].
COLLE, A ;
GUINET, R ;
LECLERCQ, M ;
MANUEL, Y .
CLINICA CHIMICA ACTA, 1976, 67 (01) :93-97
[7]   HEATED BIURET-FOLIN PROTEIN ASSAY WHICH GIVES EQUAL ABSORBANCE WITH DIFFERENT PROTEINS [J].
DORSEY, TE ;
MCDONALD, PW ;
ROELS, OA .
ANALYTICAL BIOCHEMISTRY, 1977, 78 (01) :156-164
[8]   CROSSED IMMUNOELECTROPHORESIS [J].
GANROT, PO .
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 1972, 29 :39-&
[9]   THE TRUE ENDOGENOUS CREATININE CLEARANCE [J].
HAUGEN, HN ;
BLEGEN, EM .
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 1953, 5 (01) :67-71
[10]  
HOCHWALD GM, 1967, P SOC EXP BIOL MED, V124, P961