Mucosal and systemic immunogenicity of a recombinant, non-ADP-ribosylating pertussis toxin: Effects of formaldehyde treatment

被引：21

作者：

Cropley, I

Douce, G

Roberts, M

Chatfield, S

Pizza, M

Marsili, I

Rappuoli, R

Dougan, G

机构：

[1] UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOCHEM,MEDEVA VACCINE RES UNIT,LONDON,ENGLAND

[2] UNIV GLASGOW,DEPT VET PATHOL,GLASGOW G61 1QH,LANARK,SCOTLAND

[3] INST RIC IMMUNOBIOL SIENA,I-53100 SIENA,ITALY

来源：

VACCINE | 1995年 / 13卷 / 17期

基金：

英国医学研究理事会;

关键词：

pertussis toxin; mucosal immunization; formaldehyde;

D O I：

10.1016/0264-410X(95)00134-M

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The effect of formaldehyde treatment on the mucosal and systemic immunogenicity of the genetically detoxified pertussis toxin (PT-9K/129G) was investigated Groups of BALB/c were immunized intranasally (in.) or subcutaneously (s.c.) with untreated lightly formaldehyde treated (LFT) or heavily formaldehyde treated (HFT) recombinant pertussis toxin (PT) mutant, PT-9K/129G. Intranasal immunization with native PT-9K/129G induced significant levels of anti-toxin antibodies in serum and IgA anti-toxin responses in nasal and lung lavages of these mice. Similar local and systemic responses were observed following intransal immunization with LFT toxin. However, i.n. immunization with HFT toxin failed to induce a local IgA response and elicited a much diminished anti-toxin response in the serum. In contrast, the total antibody response following s.c. immunization was not significantly affected In addition, in. immunization with native PT-9K/129G induced low but detectable levels of toxin neutralizing antibodies in the serum, These results show that native PT-9K/129G protein acts as a mucosal immunogen in mice and that this activity is greatly diminished by HFT of the protein.

引用

页码：1643 / 1648

页数：6

共 26 条

[1]

AIZAPURUA DH, 1988, J EXP MED, V167, P440

[2]

[Anonymous], 1988, LANCET, Vi, P955

[3]

Bienenstock J, 1978, Adv Exp Med Biol, V107, P53

[4] FORMALDEHYDE TREATMENT OF PROTEINS CAN CONSTRAIN PRESENTATION TO T-CELLS BY LIMITING ANTIGEN-PROCESSING [J].