A HETERONUCLEAR CORRELATION EXPERIMENT FOR SIMULTANEOUS DETERMINATION OF N-15 LONGITUDINAL DECAY AND CHEMICAL-EXCHANGE RATES OF SYSTEMS IN SLOW EQUILIBRIUM

被引：380
作者：
FARROW, NA
ZHANG, OW
FORMANKAY, JD
KAY, LE
机构：
[1] UNIV TORONTO,DEPT MOLEC & MED GENET,TORONTO M5S 1A8,ON,CANADA
[2] UNIV TORONTO,DEPT BIOCHEM & CHEM,TORONTO M5S 1A8,ON,CANADA
[3] HOSP SICK CHILDREN,DIV BIOCHEM RES,TORONTO M5G 1X8,ON,CANADA
来源：
JOURNAL OF BIOMOLECULAR NMR | 1994年 / 4卷 / 05期
关键词：
PROTEIN DYNAMICS; CHEMICAL EXCHANGE; PROTEIN FOLDING;
D O I：
10.1007/BF00404280
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A heteronuclear correlation experiment is described which permits simultaneous characterization of both N-15 longitudinal decay rates and slow conformational exchange rates. Data pertaining to the exchange between folded and unfolded forms of an SH3 domain is used to illustrate the technique. Because the unfolded form of the molecule, on average, shows significantly higher NH exchange rates than the folded form, an approach which minimizes the degree of water saturation is employed, enabling the extraction of accurate rate constants.
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页码：727 / 734
页数：8
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