THE KINASE INSERT DOMAIN OF COLONY STIMULATING FACTOR-I RECEPTOR IS DISPENSABLE FOR CSF-1 INDUCED PHOSPHATIDYLCHOLINE HYDROLYSIS

被引：20
作者：
CHOUDHURY, GG
SYLVIA, VL
WANG, LM
PIERCE, J
SAKAGUCHI, AY
机构：
[1] UNIV TEXAS, HLTH SCI CTR, DEPT CELLULAR & STRUCT BIOL, 7703 FLOYD CURL DR, SAN ANTONIO, TX 78284 USA
[2] NCI, CELLULAR & MOLEC BIOL LAB, BETHESDA, MD 20892 USA
来源：
FEBS LETTERS | 1991年 / 282卷 / 02期
关键词：
COLONY STIMULATING FACTOR-I RECEPTOR; TYROSINE KINASE; PHOSPHATIDYLCHOLINE HYDROLYSIS; NIH-3T3; CELL;
D O I：
10.1016/0014-5793(91)80511-Z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mouse NIH 3T3 fibroblasts transfected with human colony stimulating factor-1 receptor produced diacylglycerol in response to CSF1 and this correlated with elevated phosphatidylcholine hydrolyzing activity measured in an in vitro assay. Treatment of cells with the isoflavone derivative genistein attenuated PC hydrolysis in vitro suggesting a role for CSF1R tyrosine kinase activity. A CSF1R mutant lacking 67 amino acids of the kinase insert domain, which may affect the association of receptor with certain substrates, stimulated PC hydrolysis in response to CSF1. Coupling to PC hydrolysis is likely a general property of CSF1R and the kinase insert domain is dispensable for this activity.
引用
收藏
页码：351 / 354
页数：4
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