Detection of variant infectious bronchitis viruses in broiler flocks in Libya

被引：33

作者：

Awad, Faez ^{[1
,2
]}

Baylis, Matthew ^{[1
]}

Ganapathy, Kannan ^{[1
]}

机构：

[1] Univ Liverpool, Inst Infect & Global Hlth, Leahurst Campus, Neston CH64 7TE, South Wirral, England

[2] Univ Omar Al Mukhtar, Fac Vet Med, Al Bayda, Libya

来源：

INTERNATIONAL JOURNAL OF VETERINARY SCIENCE AND MEDICINE | 2014年 / 2卷 / 01期

关键词：

Chicken; Libya; Variant infectious bronchitis virus;

D O I：

10.1016/j.ijvsm.2014.01.001

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A number of broiler flocks with respiratory disease and high mortality in five broiler farms in Libya were sampled for detection of infectious bronchitis virus (IBV). Twelve IBV strains from these farms were detected by reverse transcription polymerase chain reaction (RT-PCR) and differentiated by nucleotide sequencing of the hypervariable region of the S1 gene. A pair-wise comparison of the sequences showed two distinctive patterns. Those from farms 1, 2, 4 and 5, formed a separate cluster with 94-99% relatedness to the Egyptian IBV strains CK/Eg/BSU-2/2011, CK/Eg/BSU-3/2011 and Eg/1212B. Sequences from the farm 3 formed another cluster with 100% relatedness to Eg/CLEVB-2/IBV/012 and IS/1494/06. This appears to be the first report on the co-circulation these variant IBVs in Libya. (C) 2014 Production and hosting by Elsevier B.V. on behalf of Faculty of Veterinary Medicine, Cairo University.

引用

页码：78 / 82

页数：5

共 19 条

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