QUANTITATIVE GAS CHROMATOGRAPHIC ASSAY FOR CAFFEIC ACID MOIETIES IN TOBACCO

被引：8

作者：

ANDERSEN, RA

MOEGLING, G

机构：

[1] Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Lexington

[2] Department of Agronomy, University of Kentucky, Lexington

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 27卷 / 03期

关键词：

D O I：

10.1016/0003-2697(69)90053-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for the quantitative determination of caffeic acid moieties in tobacco by gas-liquid chromatography. Caffeic acid esters are precipitated with lead acetate, recovered, and subjected to a transesterification reaction to produce the n-butyl ester of caffeic acid. After silylation of the n-butyl ester of caffeic acid the derivative, bis (trimethylsilyl) caffeoyl n-butyrate, is suitable for gas chromatography on 12% SE-30. Anthracene and pyrene were used as internal standards. Results for chlorogenic acid in tobacco samples obtained by a paper chromatographic-spectrophotometric method are comparable with results obtained by gas chromatography. © 1969.

引用

页码：397 / &

共 11 条

[1] GAS CHROMATOGRAPHY OF PHENOLIC ACIDS [J].