A COLLECTION OF CDNA CLONES WITH SPECIFIC EXPRESSION PATTERNS IN MOUSE-BRAIN

被引：77

作者：

KATO, K

机构：

[1] Mrc Molecular Genetics Unit, Cambridge, CB2 2QH, Hills Road

来源：

EUROPEAN JOURNAL OF NEUROSCIENCE | 1990年 / 2卷 / 08期

关键词：

cerebellum; in situ hybridization; Northern hybridization; random cloning;

D O I：

10.1111/j.1460-9568.1990.tb00460.x

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A total of 950 cDNA clones were randomly selected from mouse cerebellar cDNA libraries, and of these, about 130 clones were found to correspond to mRNAs which were expressed unequally between the cerebellum and other parts of mouse brain. Their distribution patterns in adult mouse brain were analysed by in situ hybridization, and eight clones were found restricted to specific regions of the brain, including four clones specific to cerebellar granule cells and one clone specific to Purkinje cells. Another 27 clones were preferentially expressed in a diverse, but distinctive subpopulation of brain cells. Among them seven clones were especially abundant in specific nuclei, and three in specific fibre bundles. These clones will be useful in defining new subpopulations of brain cells characterized by different gene expression. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：704 / 711

页数：8

共 24 条

[1] POSTNATAL-DEVELOPMENT OF CEREBELLAR CORTEX IN RAT .1. EXTERNAL GERMINAL LAYER AND TRANSITIONAL MOLECULAR LAYER [J].

ALTMAN, J .

JOURNAL OF COMPARATIVE NEUROLOGY, 1972, 145 (03) :353-+

[2] MOLECULAR PROBES FOR THE DEVELOPMENT AND PLASTICITY OF NEURAL CREST DERIVATIVES [J].

ANDERSON, DJ ;

AXEL, R .

CELL, 1985, 42 (02) :649-662

[3]

[Anonymous], 1965, CATALOG NEUROLOGICAL

[4] PURIFICATION OF BIOLOGICALLY-ACTIVE GLOBIN MESSENGER-RNA BY CHROMATOGRAPHY ON OLIGOTHYMIDYLIC-ACID-CELLULOSE [J].

AVIV, H ;

LEDER, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (06) :1408-&

[5] COMPLEXITY AND CHARACTERIZATION OF POLYADENYLATED RNA IN MOUSE-BRAIN [J].

BANTLE, JA ;

HAHN, WE .

CELL, 1976, 8 (01) :139-150

[6] MONOCLONAL-ANTIBODIES THAT LABEL DISCRETE CELL-TYPES IN THE MAMMALIAN NERVOUS-SYSTEM [J].

BARNSTABLE, CJ ;

AKAGAWA, K ;

HOFSTEIN, R ;

HORN, JP .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1983, 48 :863-876

[7] COMPLEXITY OF CYTOPLASMIC POLYADENYLATED AND NONPOLYADENYLATED RAT-BRAIN RIBONUCLEIC-ACIDS [J].

CHIKARAISHI, DM .

BIOCHEMISTRY, 1979, 18 (15) :3249-3256

[8] PROBES FOR RARE MESSENGER-RNAS REVEAL DISTRIBUTED CELL SUBSETS IN CANARY BRAIN [J].

CLAYTON, DF ;

HUECAS, ME ;

SINCLAIRTHOMPSON, EY ;

NASTIUK, KL ;

NOTTEBOHM, F .

NEURON, 1988, 1 (03) :249-261

[9] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[10] NEURONAL LOCALIZATION OF AMYLOID BETA-PROTEIN PRECURSOR MESSENGER-RNA IN NORMAL HUMAN-BRAIN AND IN ALZHEIMERS-DISEASE [J].

GOEDERT, M .

EMBO JOURNAL, 1987, 6 (12) :3627-3632

← 1 2 3 →