EXPRESSION OF FETAL-TYPE INTERMEDIATE FILAMENTS BY 17-DAY-OLD RAT SERTOLI CELLS CULTURED ON RECONSTITUTED BASEMENT-MEMBRANE

被引：15

作者：

GUILLOU, F ^{[1
]}

MONETKUNTZ, C ^{[1
]}

FONTAINE, I ^{[1
]}

FLECHON, JE ^{[1
]}

机构：

[1] INRA, F-78350 JOUY EN JOSAS, FRANCE

来源：

CELL AND TISSUE RESEARCH | 1990年 / 260卷 / 02期

关键词：

Basement membrane; Cytokeratin; Intermediate filaments; Rat (Wistar); Sertoli cells; Testis; Vimentin;

D O I：

10.1007/BF00318642

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The expression of cytokeratin- and vimentin-type intermediate filaments was studied by means of immunohistochemistry in Sertoli cells cultured on two types of reconstituted basement membrane in two-compartment culture chambers. In situ, the Sertoli cells of 17-day-old rats contained only vimentin intermediate filaments. During culture, a gradual reorganization of intermediate filaments accompanied by an increased cytokeratin immunoreactivity was observed. After 6 days, Sertoli cells contained both cytokeratin and vimentin, and the same cytokeratin type as in fetal and newborn testis was revealed by electrophoresis and immunoblotting. The present study shows that the isolation and culture of Sertoli cells causes, even in an improved culture system qualitative changes in the expression of intermediate filament proteins. © 1990 Springer-Verlag.

引用

页码：395 / 401

页数：7

共 35 条

[1] MODULATION OF SERTOLI-CELL FUNCTIONS IN THE 2-CHAMBER ASSEMBLY BY PERITUBULAR CELLS AND EXTRACELLULAR-MATRIX [J].