BIOASSAY FOR STUDYING THE ROLE OF SIDEROPHORES IN POTATO GROWTH-STIMULATION BY PSEUDOMONAS SPP IN SHORT POTATO ROTATIONS

被引：53
作者：
BAKKER, PAHM
BAKKER, AW
MARUGG, JD
WEISBEEK, PJ
SCHIPPERS, B
机构：
[1] STATE UNIV UTRECHT, DEPT MOLEC CELL BIOL, 3584 CH UTRECHT, NETHERLANDS
[2] UNIV AMSTERDAM, 3742 CP BAARN, NETHERLANDS
来源：
SOIL BIOLOGY & BIOCHEMISTRY | 1987年 / 19卷 / 04期
关键词：
D O I：
10.1016/0038-0717(87)90036-8
中图分类号：
S15 [土壤学];
学科分类号：
0903 ; 090301 ;
摘要：
A bioassay is described for studying the mechanisms of growth stimulation by fluorescent pseudomonads using rooted potato stem cuttings. Root development of potato stem cuttings was inhibited in short potato-rotation soil compared to development in long potato-rotation soil. Treatment with Pseudomonas fluorescens isolate WCS374 or Pseudomonas putida isolate WCS358 increased root development in short potato-rotation soil, whereas siderophore-negative Tn5 transposon mutants of isolate WCS358 had no effect. Both siderophore-producing and siderophore-negative Tn5 mutants of isolate WCS358 could be recovered in similar numbers from the root systems. These results were obtained in a bioassay of 8 days duration. It is postulated that failure of siderophore-negative Tn5 transposon mutants of isolate WCS358 to induce growth stimulation demonstrates that siderophore production is a prerequisite for growth stimulation in short potato-rotation soil.
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页码：443 / 449
页数：7
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