CLONING AND IMMUNOLOGICAL CHARACTERIZATION OF ECHINOCOCCUS-GRANULOSUS FERRITIN

被引：16

作者：

ERSFELD, K ^{[1
]}

CRAIG, PS ^{[1
]}

机构：

[1] UNIV MANCHESTER,DEPT BIOCHEM,MANCHESTER M13 9PT,LANCS,ENGLAND

来源：

PARASITOLOGY RESEARCH | 1995年 / 81卷 / 05期

基金：

英国惠康基金;

关键词：

D O I：

10.1007/BF00931498

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A cDNA clone of Echinococcus granulosus has been isolated from an expression library screened with sera from cystic echinococcosis patients. The deduced amino acid sequence shows 56% homology to the heavy chain of human ferritin. E. granulosus ferritin contains 173 amino acid residues and has a calculated molecular weight of 19830 Da and a statistical isoelectric point of 7.6. Functionally important amino acid residues of the ferroxidase centre are conserved in comparison with other ferritins. In vitro-translated E. granulosus ferritin was tested for its diagnostic potential by immunoprecipitation. The antigenic reactivity exhibited a good potential for the further development of E. granulosus ferritin as an immunodiagnostic tool for human hydatidosis.

引用

页码：382 / 387

页数：6

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