FINE-STRUCTURE OF THE ENDOLYMPHATIC DUCT IN THE RAT - A SCANNING AND TRANSMISSION ELECTRON-MICROSCOPY STUDY

被引：2
作者：
QVORTRUP, K [1 ]
ROSTGAARD, J [1 ]
BRETLAU, F [1 ]
机构：
[1] RIGSHOSP, DEPT OTOLARYNGOL HEAD & NECK SURG, COPENHAGEN, DENMARK
来源：
ACTA OTO-LARYNGOLOGICA | 1995年 / 115卷 / 02期
关键词：
RAT; ENDOLYMPHATIC DUCT; SCANNING ELECTRON MICROSCOPY; VASCULAR PERFUSION FIXATION;
D O I：
10.3109/00016489509139300
中图分类号：
R76 [耳鼻咽喉科学];
学科分类号：
100213 ;
摘要：
To investigate the surface morphology of the endolymphatic duct epithelium, 8 rats were vascularly perfused with glutaraldehyde in a buffered and oxygenated blood substitute. Optimal preservation of the epithelium for scanning electron microscopy was attained by coating of the specimens with OsO4 and thiocarbohydrazide followed by a continuous dehydration procedure. Three types of cells were identified with the scanning electron microscope: A polygonal and oblong epithelial cell was observed in the largest number throughout the duct, whereas in the juxta-saccular half of the duct two additional types of epithelial cells were observed. The scanning electron microscopical observations are compared and discussed with reference to transmission electron microscopical observations of the endolymphatic duel.
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页码：241 / 245
页数：5
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