EFFECT OF SUPPORT MATERIAL AND ENZYME PRETREATMENT ON ENANTIOSELECTIVITY OF IMMOBILIZED SUBTILISIN IN ORGANIC-SOLVENTS

被引：32

作者：

ORSAT, B

DRTINA, GJ

WILLIAMS, MG

KLIBANOV, AM

机构：

[1] MIT,DEPT CHEM,CAMBRIDGE,MA 02139

[2] THREE M CO,CORP RES LABS,ST PAUL,MN 55144

[3] THREE M CO,BIOSCI LABS,ST PAUL,MN 55144

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1994年 / 44卷 / 10期

关键词：

SUBTILISIN; ORGANIC SOLVENTS; IMMOBILIZED ENZYME; AQUAPHILICITY; HYDROPHILICITY; ENANTIOSELECTIVITY;

D O I：

10.1002/bit.260441015

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Subtilisin Carlsberg was covalently attached to five macroporous acrylic supports of varying aquaphilicity (a measure of hydrophilicity). Kinetic parameters of the transesterification of S and R enantiomers of secphenethyl alcohol with vinyl butyrate, catalyzed by various immobilized subtilisins, were determined in anhydrous dioxane and acetonitrile. Enzyme enantioselectivity in acetonitrile, but not in dioxane, correlated with the aquaphilicity of the support; a mechanistic rationale for this phenomenon was proposed. Although the catalytic activity of immobilized subtilisin in anhydrous solvents strongly depended on enzyme pretreatment, the enantioselectivity was essential conserved. (C) 1994 John Wiley and Sons, Inc.

引用

页码：1265 / 1269

页数：5

共 30 条

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