FC-EPSILON-RI-STIMULATED CA2+-DEPENDENT SECRETION FROM RAT BASOPHILIC LEUKEMIA (RBL-2H3) CELLS PERMEABILIZED WITH STAPHYLOCOCCAL ALPHA-TOXIN - FC-EPSILON-RI-OPERATED SIGNALS ARE NOT MIMICKED BY THE ACTIONS OF GTP-GAMMA-S

被引：4
作者：
OISHI, K
UEKUSA, T
IWASAKI, T
FUKUDA, Y
IKEYA, H
INOUE, R
UCHIDA, MK
机构：
[1] Department of Molecular Pharmacology, Meiji College of Pharmacy 1-35-23, Setagaya-ku, Tokyo, 154, Nozawa
来源：
GENERAL PHARMACOLOGY | 1994年 / 25卷 / 04期
关键词：
RBL-2H3; CELLS; SECRETION; PERMEABILIZATION; ALPHA-TOXIN; CA2+; FC-EPSILON-RI; GUANINE NUCLEOTIDE;
D O I：
10.1016/0306-3623(94)90253-4
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
1. RBL-2H3 cells permeabilized with cc-toxin responded to dinitrophenol (30-40 mol/mol)-conjugated human serum albumin, as antigen, to secrete [(14) C]serotonin in the micromolar range of free Ca2+. 2. Calcium ion alone did not cause substantial secretion. 3. Guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) (100 mu M) in combination with Ca2+ produced only negligible [C-14]serotonin secretion. 4. GTP gamma S, in the presence of cytochalasin D, caused optimal secretion of [C-14]serotonin in a Ca2+-dependent manner.
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收藏
页码：729 / 737
页数：9
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