AUTOANTIBODIES AGAINST VIMENTIN IN A MURINE MODEL OF MYOCARDITIS

被引：17

作者：

SATO, Y ^{[1
]}

MATSUMORI, A ^{[1
]}

SASAYAMA, S ^{[1
]}

机构：

[1] KYOTO UNIV,FAC MED,DEPT INTERNAL MED,DIV 3,SAKYO KU,KYOTO 606,JAPAN

来源：

AUTOIMMUNITY | 1994年 / 18卷 / 02期

关键词：

VIMENTIN; INTERMEDIATE FILAMENT; ANTIENDOTHELIAL CELL ANTIBODY; VIRAL MYOCARDITIS;

D O I：

10.3109/08916939409007988

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The presence of autoantibodies in the sera of patients with myocarditis has suggeted that autoimmunity is a sequela of myocarditis. In this study, we examined anti-vimentin antibodies in a murine model of myocarditis. Four-week-old male DBA/2 mice were inoculated intraperitoneally with 10 plaque-forming units of encephalomyocarditis (EMC) virus. The surviving mice were killed on Days 0 (n = 7), 2 (n = 6), 5 (n = 6), 7 (n = 6), 9 (n = 6), 14 (n = 5), 60 (n = 6) and 540 (n = 3) after virus inoculation. The presence of anti-vimenlin autoantibodies in the sera of mice was determined by ELISA. The optical density (OD) of anti-vimentin IgG and IgM autoantibodies of day 0 mice was 0.03 +/- 0.03 and 0.12 +/- 0.08, respectively. when positive antibody was defined as over the mean value plus 2-standard deviations of the sera of day 0 mice, anti-vimentin IgG antibodies were negative before day 5 but positive in all mice on day 9. Both IgG and IgM OD of the sera from day 9 mice (IgG; 0.26 +/- 0.12, IgM; 0.30 +/- 0.08) were significantly higher than those of the sera from day 0 mice (p < 0.005) and decreased thereafter on day 14. In the chronic stage, five of six mice were positive on day 60 and two of three mice were positive on day 540. Although further studies are needed to elucidate the pathogenetic role of anti-vimentin antibodies, these antibodies may be a parameter of the interstitial injury.

引用

页码：145 / 148

页数：4

共 19 条

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