BLAI AND BLAR1 REGULATE BETA-LACTAMASE AND PBP 2A PRODUCTION IN METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS

被引:122
作者
HACKBARTH, CJ
CHAMBERS, HF
机构
[1] UNIV CALIF SAN FRANCISCO, SAN FRANCISCO, CA 94143 USA
[2] SAN FRANCISCO GEN HOSP, SAN FRANCISCO, CA 94110 USA
关键词
D O I
10.1128/AAC.37.5.1144
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
For Staphylococcus aureus, it is hypothesized that two genes located upstream of the beta-lactamase gene, blaZ, are required for the inducible expression of beta-lactamase. blaR1 is predicted to encode a signal-transducing membrane protein, and blaI is predicted to encode a repressor protein. These same two genes may also regulate the production of penicillin-binding protein 2a (PBP 2a), a protein essential for expression of methicillin resistance. To confirm that these two genes encode products that can control both beta-lactamase and PBP 2a production, blaI, blaR1, and blaZ with a 150-nucleotide deletion at the 3' end were subcloned from a 30-kb staphylococcal beta-lactamase plasmid and three beta-lactamase-negative strains of methicillin-resistant S. aureus were transformed with the recombinant plasmid containing that insert. The production of PBP 2a and a nonfunctional beta-lactamase was detected by fluorography and by immunoblots with polyclonal antisera directed against each of the proteins. Whereas the parent strains did not produce beta-lactamase and constitutively produced PBP 2a, PBP 2a and a truncated beta-lactamase were now inducible in the transformants. Therefore, two plasmid-derived genes regulate the production of both PBP 2a and beta-lactamase.
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页码:1144 / 1149
页数:6
相关论文
共 40 条
[1]  
AUGUSTIN J, 1990, FEMS MICROBIOL LETT, V66, P203, DOI 10.1016/0378-1097(90)90283-V
[2]  
Ausubel F, 1988, CURRENT PROTOCOLS MO
[3]  
Bolivar F, 1979, Methods Enzymol, V68, P245
[4]   A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI [J].
BOYER, HW ;
ROULLAND.D .
JOURNAL OF MOLECULAR BIOLOGY, 1969, 41 (03) :459-&
[5]   EFFECT OF NACL AND NAFCILLIN ON PENICILLIN-BINDING PROTEIN-2A AND HETEROGENEOUS EXPRESSION OF METHICILLIN RESISTANCE IN STAPHYLOCOCCUS-AUREUS [J].
CHAMBERS, HF ;
HACKBARTH, CJ .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1987, 31 (12) :1982-1988
[6]   INCREASED AMOUNTS OF A NOVEL PENICILLIN-BINDING PROTEIN IN A STRAIN OF METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS EXPOSED TO NAFCILLIN [J].
CHAMBERS, HF ;
HARTMAN, BJ ;
TOMASZ, A .
JOURNAL OF CLINICAL INVESTIGATION, 1985, 76 (01) :325-331
[7]   BINDING-AFFINITY FOR PENICILLIN-BINDING PROTEIN-2A CORRELATES WITH INVIVO ACTIVITY OF BETA-LACTAM ANTIBIOTICS AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS [J].
CHAMBERS, HF ;
SACHDEVA, M ;
KENNEDY, S .
JOURNAL OF INFECTIOUS DISEASES, 1990, 162 (03) :705-710
[8]  
EAST AK, 1989, J GEN MICROBIOL, V135, P1001
[9]   COMPARISON OF CONVENTIONAL SUSCEPTIBILITY TESTS WITH DIRECT DETECTION OF PENICILLIN-BINDING PROTEIN-2A IN BORDERLINE OXACILLIN-RESISTANT STRAINS OF STAPHYLOCOCCUS-AUREUS [J].
GERBERDING, JL ;
MIICK, C ;
LIU, HH ;
CHAMBERS, HF .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1991, 35 (12) :2574-2579
[10]   LOW-AFFINITY PENICILLIN-BINDING PROTEIN ASSOCIATED WITH BETA-LACTAM RESISTANCE IN STAPHYLOCOCCUS-AUREUS [J].
HARTMAN, BJ ;
TOMASZ, A .
JOURNAL OF BACTERIOLOGY, 1984, 158 (02) :513-516