UMBILICAL-CORD BLOOD AS A SOURCE OF CD34 POSITIVE HEMATOPOIETIC STEM-CELLS

被引：0

作者：

RAO, SGA

DESAI, SS

GOUD, AP

DEORA, A

AMIN, MK

GANGAL, SG

机构：

[1] TATA MEM HOSP, CANC RES INST, DIV CELL & DEV PATHOL, BOMBAY 400012, MAHARASHTRA, INDIA

[2] TATA MEM HOSP, CANC RES INST, DIV IMMUNOL, BOMBAY 400012, MAHARASHTRA, INDIA

来源：

INDIAN JOURNAL OF MEDICAL RESEARCH | 1995年 / 101卷

关键词：

BONE; MARROW TRANSPLANTATION; HEMATOPOIESIS; STEM CELLS;

D O I：

暂无

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this paper we have used a monoclonal antibody to CD34 an antigen expressed solely on stem cells, and stem cell colony assays to show that umbilical cord blood has nearly the same number of functional stem cells as compared to normal bone-marrow. The number of CD34+ve cells in cord blood being 2 to 2.7 per cent, whereas bone-marrow had 3 to 3.5 per cent. The multi-potent colony forming cells (CFU-GEMM) were 60+/-18 in cord blood per 2x10(5) mononuclear cells (MNCs), whereas normal bone-marrow had 70+/-10 per 2x10(5) MNCs. Enrichment of these stem cells on Percoll gradients was successful for normal bone-marrow but not for cord blood.

引用

页码：28 / 30

页数：3