LETS GET SPECIFIC - THE RELATIONSHIP BETWEEN SPECIFICITY AND AFFINITY

被引：168
作者：
EATON, BE
GOLD, L
ZICHI, DA
机构：
来源：
CHEMISTRY & BIOLOGY | 1995年 / 2卷 / 10期
关键词：
D O I：
10.1016/1074-5521(95)90023-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The factors that lead to high-affinity binding are a good fit between the surfaces of the two molecules in their ground state and charge complementarity. Exactly the same factors give high specificity for a target. We argue that selection for high-affinity binding automatically leads to highly specific binding. This principle can be used to simplify screening approaches aimed at generating useful drugs.
引用
收藏
页码：633 / 638
页数：6
相关论文
共 26 条
[1]   THE FGF FAMILY OF GROWTH-FACTORS AND ONCOGENES [J].
BASILICO, C ;
MOSCATELLI, D .
ADVANCES IN CANCER RESEARCH, 1992, 59 :115-165
[2]  
CHEN H, 1995, IN PRESS METHODS ENZ
[3]   3 SMALL RIBOOLIGONUCLEOTIDES WITH SPECIFIC ARGININE SITES [J].
CONNELL, GJ ;
ILLANGESEKARE, M ;
YARUS, M .
BIOCHEMISTRY, 1993, 32 (21) :5497-5502
[4]   STEREOSPECIFIC RECOGNITION OF TRYPTOPHAN AGAROSE BY INVITRO SELECTED RNA [J].
FAMULOK, M ;
SZOSTAK, JW .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (10) :3990-3991
[5]   OLIGONUCLEOTIDES AS RESEARCH, DIAGNOSTIC, AND THERAPEUTIC AGENTS [J].
GOLD, L .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (23) :13581-13584
[6]   DIVERSITY OF OLIGONUCLEOTIDE FUNCTIONS [J].
GOLD, L ;
POLISKY, B ;
UHLENBECK, O ;
YARUS, M .
ANNUAL REVIEW OF BIOCHEMISTRY, 1995, 64 :763-797
[7]  
Gold L., 1993, RNA WORLD, P497
[8]   NUCLEASE-RESISTANT NUCLEIC-ACID LIGANDS TO VASCULAR-PERMEABILITY FACTOR VASCULAR ENDOTHELIAL GROWTH-FACTOR [J].
GREEN, LS ;
JELLINEK, D ;
BELL, C ;
BEEBE, LA ;
FEISTNER, BD ;
GILL, SC ;
JUCKER, FM ;
JANJIC, N .
CHEMISTRY & BIOLOGY, 1995, 2 (10) :683-695
[9]   INHIBITION OF RECEPTOR-BINDING BY HIGH-AFFINITY RNA LIGANDS TO VASCULAR ENDOTHELIAL GROWTH-FACTOR [J].
JELLINEK, D ;
GREEN, LS ;
BELL, C ;
JANJIC, N .
BIOCHEMISTRY, 1994, 33 (34) :10450-10456
[10]   HIGH-AFFINITY RNA LIGANDS TO BASIC FIBROBLAST GROWTH-FACTOR INHIBIT RECEPTOR-BINDING [J].
JELLINEK, D ;
LYNOTT, CK ;
RIFKIN, DB ;
JANJIC, N .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (23) :11227-11231
123