ALKALINE-PHOSPHATASE ACTIVITY IN THE HUMAN PROMYELOCYTIC LEUKEMIA-CELL LINE, HL-60, INDUCED BY RETINOIC ACID AND RECOMBINANT HUMAN GRANULOCYTE-COLONY-STIMULATING FACTOR

被引：3

作者：

TAOKA, T

YAMAOKA, G

ARAI, T

KIUCHI, H

TANAKA, T

TAKAHARA, J

KAWANISHI, K

IRINO, S

机构：

[1] KAGAWA MED SCH,DEPT CLIN LAB,1750-1 IKENOBE,MIKI,KAGAWA 76107,JAPAN

[2] KAGAWA MED SCH,DEPT INTERNAL MED 1,MIKI,KAGAWA 76107,JAPAN

来源：

LEUKEMIA RESEARCH | 1993年 / 17卷 / 08期

关键词：

DIFFERENTIATION; ALKALINE PHOSPHATASE; HL-60; RETINOIC ACID; RHG-CSF; PROTEIN KINASE-C;

D O I：

10.1016/0145-2126(93)90076-W

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Recombinant human granulocyte colony-stimulating factor (rhG-CSF) not only enhanced the growth of HL-60 cells, but also significantly increased NBT-reducing ability and alkaline phosphatase (ALP) activity of the cells, which were enhanced by the treatment with retinoic acid (RA). Protein kinase C inhibitors (H-7 and staurosporine) significantly suppressed this induction of ALP. The pretreatment with RA followed by rhG-CSF treatment showed almost the same degree of ALP activity as that induced by the simultaneous treatment with RA and rhG-CSF. This study suggests that RA and rhG-CSF are the potent inducers of ALP activity of HL-60 cells and protein kinase C is supposed to have a role in this induction of ALP.

引用

页码：695 / 698

页数：4

共 6 条

[1] NORMAL FUNCTIONAL-CHARACTERISTICS OF CULTURED HUMAN PROMYELOCYTIC LEUKEMIA-CELLS (HL-60) AFTER INDUCTION OF DIFFERENTIATION BY DIMETHYLSULFOXIDE [J].