OPTIMIZATION OF PH FOR THE SEPARATION OF ORGANIC-ACIDS IN CAPILLARY ZONE ELECTROPHORESIS

被引：107

作者：

SMITH, SC ^{[1
]}

KHALEDI, MG ^{[1
]}

机构：

[1] N CAROLINA STATE UNIV,DEPT CHEM,BOX 8204,RALEIGH,NC 27695

来源：

ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 03期

关键词：

D O I：

10.1021/ac00051a001

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The migration behavior and optimum separation of a set of substituted phenols is presented. A mathematical model that describes mobility in CZE in terms of fundamental constants of each solute (acid dissociation constant K(a), mobility of the dissociated acid mu(A)-) and the pH of the buffer was used to predict the mobility of each solute as a function of pH. The resolution between each peak pair was calculated, and a window diagram of minimum resolution as a function of pH was employed in determining the optimum pH. A comparison of the predicted and actual electropherograms shows the usefulness of this technique.

引用

页码：193 / 198

页数：6

共 27 条

[1]

Ahuja S., 1989, SELECTIVITY DETECTAB

[2] HIGH-PERFORMANCE CAPILLARY ELECTROPHORETIC SEPARATION OF BASES, NUCLEOSIDES, AND OLIGONUCLEOTIDES - RETENTION MANIPULATION VIA MICELLAR SOLUTIONS AND METAL ADDITIVES [J].

COHEN, AS ;

TERABE, S ;

SMITH, JA ;

KARGER, BL .

ANALYTICAL CHEMISTRY, 1987, 59 (07) :1021-1027

[3] SEPARATION OF OPTICAL ISOMERS BY CAPILLARY ZONE ELECTROPHORESIS BASED ON HOST GUEST COMPLEXATION WITH CYCLODEXTRINS [J].

FANALI, S .

JOURNAL OF CHROMATOGRAPHY, 1989, 474 (02) :441-446

[4]

FUJIWARA S, 1988, J CHROMATOGR, V447, P133, DOI 10.1016/S0021-9673(01)91462-1

[5] GENERATION OF VARIANCE, THEORETICAL PLATES, RESOLUTION, AND PEAK CAPACITY IN ELECTROPHORESIS AND SEDIMENTATION [J].

GIDDINGS, JC .

SEPARATION SCIENCE, 1969, 4 (03) :181-&

[6] ELECTROKINETIC RESOLUTION OF AMINO-ACID ENANTIOMERS WITH COPPER(II) ASPARTAME SUPPORT ELECTROLYTE [J].

GOZEL, P ;

GASSMANN, E ;

MICHELSEN, H ;

ZARE, RN .

ANALYTICAL CHEMISTRY, 1987, 59 (01) :44-49

[7] USE OF COMPLEXING AGENTS FOR SELECTIVE SEPARATION IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS - CHIRAL RESOLUTION VIA CYCLODEXTRINS INCORPORATED WITHIN POLYACRYLAMIDE-GEL COLUMNS [J].

GUTTMAN, A ;

PAULUS, A ;

COHEN, AS ;

GRINBERG, N ;

KARGER, BL .

JOURNAL OF CHROMATOGRAPHY, 1988, 448 (01) :41-53

[8] TABLE OF ISOTACHOPHORETIC INDEXES .1. SIMULATED QUALITATIVE AND QUANTITATIVE INDEXES OF 287 ANIONIC SUBSTANCES IN THE RANGE PH 3-10 [J].

HIROKAWA, T ;

NISHINO, M ;

AOKI, N ;

KISO, Y ;

SAWAMOTO, Y ;

YAGI, T ;

AKIYAMA, J .

JOURNAL OF CHROMATOGRAPHY, 1983, 271 (02) :D1-D106

[9] USE OF CYCLODEXTRINS IN ISOTACHOPHORESIS .3. PURITY CONTROL OF NAFTIDROFURYL HYDROGENOXALATE AND SOME OF ITS SYNTHESIS INTERMEDIATES [J].

JELINEK, I ;

DOHNAL, J ;

SNOPEK, J ;

SMOLKOVAKEULEMANSOVA, E .

JOURNAL OF CHROMATOGRAPHY, 1988, 435 (03) :496-500

[10] CAPILLARY ZONE ELECTROPHORESIS [J].

JORGENSON, JW ;

LUKACS, KD .

SCIENCE, 1983, 222 (4621) :266-272

← 1 2 3 →