FREE-RADICAL YIELDS FROM THE HOMOLYSIS OF PEROXYNITROUS ACID

被引：82

作者：

YANG, G

CANDY, TEG

BOARO, M

WILKIN, HE

JONES, P

NAZHAT, NB

SAADALLANAZHAT, RA

BLAKE, DR

机构：

[1] UNIV NEWCASTLE UPON TYNE,DEPT CHEM,NEWCASTLE TYNE NE1 7RU,TYNE & WEAR,ENGLAND

[2] UNIV LONDON LONDON HOSP,COLL MED,INFLAMMAT RES GRP,LONDON E1 2AD,ENGLAND

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1992年 / 12卷 / 04期

关键词：

NITRIC OXIDE; SUPEROXIDE; PEROXYNITRITE; HYDROXYL RADICAL; ENDOTHELIAL INJURY; FREE RADICALS;

D O I：

10.1016/0891-5849(92)90120-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recent study 1 reports discordant results for maximal free-radical yields from the decomposition of peroxynitrous acid using different assay reagents and a puzzling decrease (to zero) of the radical yields with increasing pH. An assay method using 2,2,'azino-bis(3-ethyl-benzthiazoline sulphonate) (ABTS) has been tested using stopped-flow kinetic studies, and the results imply that the assay is satisfactory when [HOONO] much greater than [-OONO] but that reactions involving peroxynitrite anion interfere at higher pH. Evidence is presented that peroxynitrite was an interfering species in the earlier studies.

引用

页码：327 / 330

页数：4