DETECTION OF VIRUS AND CELLULAR-DETERMINED ANTIGENS INSITU USING [PROTEIN-A-I-125 AND AUTO-RADIOGRAPHY

被引：6

作者：

MOAR, MH ^{[1
]}

ROSEN, A ^{[1
]}

KLEIN, G ^{[1
]}

机构：

[1] KAROLINSKA INST, DEPT TUMOR BIOL, S-10401 STOCKHOLM 60, SWEDEN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 30卷 / 03期

关键词：

D O I：

10.1016/0022-1759(79)90096-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present paper describes the use of [125I]Protein A, isolated from Staphylococcus aureus, in detecting antigen-antibody complexes by autoradiography on single cells. The method is relatively quick, reproducible, potentially more sensitive than immunofluorescence, and should be useful in combination with conventional radioimmuno-assays. We have used it to detect the cellular expression of IgM, kappa, lambda, and β2-microglobulin, as well as the expression of Epstein-Barr Virus (EBV)-associated antigens expressed in human lymphoblastoid cell lines. © 1979.

引用

页码：219 / 229

页数：11

共 19 条

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