SPECTROPHOTOMETRIC ASSAY FOR THE DETECTION OF 2-AMINOPHENOLS IN BIOLOGICAL SAMPLES

被引：6

作者：

CNUBBEN, NHP ^{[1
]}

BLAAUBOER, B ^{[1
]}

JUYN, S ^{[1
]}

VERVOORT, J ^{[1
]}

RIETJENS, IMCM ^{[1
]}

机构：

[1] UNIV UTRECHT,TOXICOL RES INST,3508 TD UTRECHT,NETHERLANDS

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 220卷 / 01期

关键词：

D O I：

10.1006/abio.1994.1314

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and efficient spectrophotometric assay is described for the determination of 2-aminophenols in biological samples, Using ferric ions as a metal catalyst, 2-aminophenol dimerizes in an acidic aqueous environment to 2-hydroxyisophenoxazin-3-one, an intensively colored dye. The newly developed assay is suitable for the detection of a-aminophenols in the micromolar range. The paper demonstrates that this chemical assay is also applicable for the determination of 2-aminophenols substituted with a halogen at the ortho, meta, or para position of the aromatic ring with respect to the amino moiety, lambda(max), and the molar extinction coefficient varying with the substituent pattern. Results obtained for fluorinated 2-aminophenol detection in biological samples by either this new method or F-19 NMR are similar. This observation corroborates that the newly developed assay is suitable for detection of 2-aminophenols in biological samples. (c) 1994 Academic Press, Inc.

引用

页码：165 / 171

页数：7

共 25 条

[1] THE METABOLISM OF 3-CHLORO-4-FLUORO-ANILINE IN DOG AND RAT [J].

BALDWIN, MK ;

HUTSON, DH .

XENOBIOTICA, 1980, 10 (02) :135-144

[2] REACTION OF ORTHO-AMINOPHENOL AND PARA-BENZOQUINONE IN ACETIC-ACID [J].

BOLOGNESE, A ;

SCHERILLO, G ;

SCHAFER, W .

JOURNAL OF HETEROCYCLIC CHEMISTRY, 1986, 23 (04) :1003-1006

[3]

BRODIE BB, 1948, J PHARMACOL EXP THER, V94, P22

[4]

BUTENANDT A, 1954, LIEBIGS ANN CHEM, V588, P106

[5] THE CHEMISTRY OF MOULD METABOLITES .2. A PARTIAL STRUCTURE FOR POLYSTICTIN [J].

CAVILL, GWK ;

CLEZY, PS ;

TETAZ, JR .

JOURNAL OF THE CHEMICAL SOCIETY, 1957, (JUN) :2646-2651

[6] STRUCTURE AND BEHAVIOR OF ORGANIC ANALYTICAL REAGENTS .2. STABILITY OF CHELATES OF ORTHO-AMINOPHENOL AND OF ORTHO-AMINOBENZENETHIOL [J].

CHARLES, RG ;

FREISER, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1952, 74 (06) :1385-1387

[7] STUDY ON THE REGIOSELECTIVITY AND MECHANISM OF THE AROMATIC HYDROXYLATION OF MONOFLUOROANILINES [J].

CNUBBEN, NHP ;

VERVOORT, J ;

VEEGER, C ;

RIETJENS, IMCM .

CHEMICO-BIOLOGICAL INTERACTIONS, 1992, 85 (2-3) :151-172

[8] On the oxydation products of o-aminophenoles. [J].

Diepolder, E .

BERICHTE DER DEUTSCHEN CHEMISCHEN GESELLSCHAFT, 1902, 35 :2816-2822

[9] 2,4-DIFLUOROANILINE AND 4-FLUOROANILINE EXPOSURE - MONITORING BY METHEMOGLOBIN AND URINE ANALYSES [J].

EADSFORTH, CV ;

LOGAN, CJ ;

MORRISON, BJ ;

WARBURTON, PA .

INTERNATIONAL ARCHIVES OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH, 1984, 54 (03) :223-232

[10]

EHLHARDT WJ, 1991, DRUG METAB DISPOS, V19, P366

← 1 2 3 →