DETECTION OF HUMAN-CHROMOSOMES IN SOMATIC-CELL HYBRIDS BY PCR ANALYSIS

被引：4
|
作者：
TRIPPUTI, P
BACCIOCCHI, G
ACCOLLA, RS
MANTERO, G
DEBELLIS, G
MANONI, M
CASSANI, G
CORNEO, G
机构：
[1] UNIV MILAN,MED CLIN,MONZA,ITALY
[2] UNIV VERONA,IST SCI IMMUNOL,I-37100 VERONA,ITALY
[3] CNR,ITBA,I-20133 MILAN,ITALY
[4] CONSORZIO BIOTECNOL,BRESCIA,ITALY
[5] TECNOGEN,MILAN,ITALY
来源：
CYTOGENETICS AND CELL GENETICS | 1993年 / 62卷 / 01期
关键词：
D O I：
10.1159/000133432
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The detection of human chromosomes in somatic cell hybrids is usually made by chromosomal analysis, Southern blot analysis with human probes, and starch-gel electrophoresis of isoenzymes. We describe here a new, quick, and very efficient method to detect human chromosomes in somatic cell hybrids between hu man and rodent (rat and mouse) cells. The method is based on the polymerase chain reaction to promote amplification of human DNA. using primers derived from localized genes or DNA fragments from each human chromosome.
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页码：1 / 4
页数：4
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